An aerobic, Gram-negative bacterial strain, TH-G33, was isolated from freshwater sediment of Taihu Lake in China. The taxonomy of strain TH-G33 was studied by using phenotypic and phylogenetic methods. Cells of strain TH-G33 were coccoid rods or rods and formed colourless to pale-pink colonies on nutrient agar. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that strain TH-G33 was related to (94·4 %), subsp. (94·1 %), subsp. (94·8 %) and (93·9 %). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 71·9 mol%. Thus, strain TH-G33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TH-G33 (=CGMCC 1.3617=JCM 13283).


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  1. Bibashi, E., Sofianou, D., Kontopoulou, K., Mitsopoulos, E. & Kokolina, E.(2000). Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 38, 456–457. [Google Scholar]
  2. Cohen, M. F., Han, X. Y. & Mazzola, M.(2004). Molecular and physiological comparison of Azospirillum spp. isolated from Rhizoctonia solani mycelia, wheat rhizosphere, and human skin wounds. Can J Microbiol 50, 291–297.[CrossRef] [Google Scholar]
  3. Eiler, A. & Bertilsson, S.(2004). Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ Microbiol 6, 1228–1243.[CrossRef] [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Han, X. Y., Pham, A. S., Tarrand, J. J., Rolston, K. V., Helsel, L. O. & Levett, P. N.(2003). Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. Am J Clin Pathol 120, 256–264.[CrossRef] [Google Scholar]
  6. Hugh, R. & Leifson, E.(1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66, 24–26. [Google Scholar]
  7. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  8. Rihs, J. D., Brenner, D. J., Weaver, R. E., Steigerwalt, A. G., Hollis, D. G. & Yu, V. L.(1993).Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31, 3275–3283. [Google Scholar]
  9. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  10. Sandoe, J. A. T., Malnick, H. & Loudon, K. W.(1997). A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 35, 2150–2152. [Google Scholar]
  11. September, S. M., Brozel, V. S. & Venter, S. N.(2004). Diversity of nontuberculoid Mycobacterium species in biofilms of urban and semiurban drinking water distribution systems. Appl Environ Microbiol 70, 7571–7573.[CrossRef] [Google Scholar]
  12. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M.(1966). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef] [Google Scholar]
  13. Subudhi, C. P. K., Adedeji, A., Kaufmann, M. E., Lucas, G. S. & Kerr, J. R.(2001). Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. Clin Microbiol Infect 7, 573–575.[CrossRef] [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  15. Wallace, P. L., Hollis, D. G., Weaver, R. E. & Moss, C. W.(1990). Biochemical and chemical characterization of pink-pigmented oxidative bacteria. J Clin Microbiol 28, 689–693. [Google Scholar]
  16. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J.(2003).Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef] [Google Scholar]

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vol. , part 1, pp. 25 - 28

Cellular fatty acid profile of sp. nov. TH-G33 . [PDF}(19 KB)

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