Symptoms of general stunting and yellowing of leaves were observed in diseased cultivated strawberry (× Duchesne) in Lithuania. Analysis of 16S rRNA gene sequences amplified by PCR indicated that the symptoms were associated with infection by a phytoplasma, designated strawberry yellows (StrawY) phytoplasma. Phylogenetic analysis of 16S rRNA gene sequences indicated that StrawY phytoplasma, ‘ Phytoplasma australiense’, ‘ Phytoplasma asteris’, stolbur phytoplasma and ‘ Phytoplasma japonicum’ shared a common ancestor, but were mutually distinct. Nucleotide sequence alignments of a 1·3 kb 16S rRNA gene sequence fragment revealed that StrawY phytoplasma shared 97·4 % or less similarity with previously described ‘ Phytoplasma’ species. These results, in addition to natural host and geographical occurrence, support the recognition of StrawY phytoplasma as a representative of a novel taxon, ‘ Phytoplasma fragariae’.


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vol. , part 1, pp. 277 - 281

A comparison of nucleotide sequences of unique regions in the 16S rRNA gene sequence of ‘ Phytoplasma fragariae’ with corresponding sequences in other ‘ Phytoplasma’ species. [PDF](64 KB)

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