Two novel species belonging to the genus are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16S rRNA and gene sequences and levels of DNA–DNA hybridization. A total of 47 strains belonging to two novel Gram-negative, psychrotolerant, HS-producing bacterial species were isolated from marine fish (cod and flounder) caught from the Baltic Sea off Denmark. The phenotypic characteristics of strains belonging to group 1 (14 strains) indicated that these represented a non-sucrose-assimilating variant of with a DNA G+C content of 47·0 mol%. Strains of group 2 (33 isolates) did not utilize the carbon substrates assimilated by except gluconate, -acetylglucosamine and malate. Their DNA G+C content was 44·0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus . Group 1 strains showed greatest sequence similarity to ATCC 8071 (99·0 %) and with NCTC 10375 (98·3 %). However, gene sequence analysis showed these isolates to share only 90·0 % sequence similarity with ATCC 8071 and 93·9 % with NCTC 10375. Similarly, DNA–DNA hybridization experiments revealed DNA relatedness levels of 38 % between the group 1 isolates and ATCC 8071 and 43 % with NCTC 10375. The group 2 strains shared less than 97 % 16S rRNA gene sequence similarities with recognized species. Comparisons between the two novel species indicated 16S rRNA gene sequence similarity of ∼98 %, gene sequence similarity of ∼89 % and DNA–DNA reassociation values of 20–34 %. Based on the evidence presented, two novel species, sp. nov. (type strain P010=ATCC BAA-1207=NBRC 100975) and sp. nov. (type strain U1417=ATCC BAA-1205=NBRC 100978), are described.


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