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Volume 56, Issue 1

sp. nov. and sp. nov., isolated from marine fish of the Baltic Sea Free

Abstract

Two novel species belonging to the genus are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16S rRNA and gene sequences and levels of DNA–DNA hybridization. A total of 47 strains belonging to two novel Gram-negative, psychrotolerant, HS-producing bacterial species were isolated from marine fish (cod and flounder) caught from the Baltic Sea off Denmark. The phenotypic characteristics of strains belonging to group 1 (14 strains) indicated that these represented a non-sucrose-assimilating variant of with a DNA G+C content of 47·0 mol%. Strains of group 2 (33 isolates) did not utilize the carbon substrates assimilated by except gluconate, -acetylglucosamine and malate. Their DNA G+C content was 44·0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus . Group 1 strains showed greatest sequence similarity to ATCC 8071 (99·0 %) and with NCTC 10375 (98·3 %). However, gene sequence analysis showed these isolates to share only 90·0 % sequence similarity with ATCC 8071 and 93·9 % with NCTC 10375. Similarly, DNA–DNA hybridization experiments revealed DNA relatedness levels of 38 % between the group 1 isolates and ATCC 8071 and 43 % with NCTC 10375. The group 2 strains shared less than 97 % 16S rRNA gene sequence similarities with recognized species. Comparisons between the two novel species indicated 16S rRNA gene sequence similarity of ∼98 %, gene sequence similarity of ∼89 % and DNA–DNA reassociation values of 20–34 %. Based on the evidence presented, two novel species, sp. nov. (type strain P010=ATCC BAA-1207=NBRC 100975) and sp. nov. (type strain U1417=ATCC BAA-1205=NBRC 100978), are described.

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Keyword(s): TMAO, trimethylamine oxide
© SGM
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2006-01-01
2024-04-19
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Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea
Int J Syst Evol Microbiol 56, 243 (2006); https://doi.org/10.1099/ijs.0.63931-0
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