1887

Abstract

A taxonomic study was performed on 13 bacterial strains isolated from preputial swabs of European bison () bulls suffering from balanoposthitis. The isolates were Gram-positive, non-motile, facultatively anaerobic, diphtheroid-shaped cells. Based on biochemical profiles and BOX-PCR-generated genomic fingerprints, the isolates were grouped into two clusters represented by four and nine strains, respectively. Strains 1(W3/01) and 2(W106/04), selected as representatives of the two clusters, shared 97·2 % 16S rRNA gene sequence similarity. The highest gene sequence similarities found (95·5–96·4 %) were to DSM 20630 and DSM 9152, demonstrating that the novel strains are members of the genus , but are not members of a recognized species. The polar lipid profiles of the two novel strains displayed the major characteristics also found in DSM 20630 and DSM 20595. Detection of a quinone system with MK-10(H) as the predominant compound confirmed phylogenetic relatedness of the novel strains to and separated them from the type species of the genus, , which contains MK-9(H) as the predominant quinone. Results from DNA–DNA hybridizations clearly demonstrated that strains 1(W3/01) and 2(W106/04) represent separate species. Based on these data, two novel species of the genus are described, for which the names sp. nov. [type strain 1(W3/01)=DSM 17162=NCTC 13354] and sp. nov. [type strain 2(W106/04)=DSM 17163=NCTC 13355] are proposed.

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2006-04-01
2020-12-03
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