1887

Abstract

A taxonomic study was performed on 13 bacterial strains isolated from preputial swabs of European bison () bulls suffering from balanoposthitis. The isolates were Gram-positive, non-motile, facultatively anaerobic, diphtheroid-shaped cells. Based on biochemical profiles and BOX-PCR-generated genomic fingerprints, the isolates were grouped into two clusters represented by four and nine strains, respectively. Strains 1(W3/01) and 2(W106/04), selected as representatives of the two clusters, shared 97·2 % 16S rRNA gene sequence similarity. The highest gene sequence similarities found (95·5–96·4 %) were to DSM 20630 and DSM 9152, demonstrating that the novel strains are members of the genus , but are not members of a recognized species. The polar lipid profiles of the two novel strains displayed the major characteristics also found in DSM 20630 and DSM 20595. Detection of a quinone system with MK-10(H) as the predominant compound confirmed phylogenetic relatedness of the novel strains to and separated them from the type species of the genus, , which contains MK-9(H) as the predominant quinone. Results from DNA–DNA hybridizations clearly demonstrated that strains 1(W3/01) and 2(W106/04) represent separate species. Based on these data, two novel species of the genus are described, for which the names sp. nov. [type strain 1(W3/01)=DSM 17162=NCTC 13354] and sp. nov. [type strain 2(W106/04)=DSM 17163=NCTC 13355] are proposed.

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2006-04-01
2024-12-08
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Bernard K. A., Bellefeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods. J Clin Microbiol 29:83–89
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Jones D., Schofield G. M. 1982; Reclassification of ‘ Corynebacterium haemolyticum ’ (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen.nov. as Arcanobacterium haemolyticum nom. rev., comb. nov. J Gen Microbiol 128, 1279–1281
  5. Collins M. D., Jones D., Schofield G. M. 1983; Arcanobacterium haemolyticum nom. rev., comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 10. Int J Syst Bacteriol 33438–440 [CrossRef]
    [Google Scholar]
  6. Jakob W., Schröder H. D., Rudolph M., Krasinski Z. A., Krasinska M., Wolf O., Lange A., Cooper J. E., Frölich K. 2000; Necrobacillosis in free-living male European bison in Poland. J Wildl Dis 36:248–256 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Buczolits S., Albrecht A., Busse H.-J., Stackebrandt E. 2003; Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53:893–896 [CrossRef]
    [Google Scholar]
  9. Kita J., Dziaba K., Piusinski W. 8 other authors 1994; Preliminary studies on the diagnosis of a disease of the genital organs of male European bison in the Bialowieza Forest of Poland. IUCN Species Surviv Comm Vet Spec Group Newsl 8:8–9
    [Google Scholar]
  10. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  11. Louws F. J., Fulbright D. W., Stephens C. T., de Bruijn F. J. 1994; Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 60:2286–2295
    [Google Scholar]
  12. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  13. Quinn P. J., Carter M. E., Markey B., Carter G. R. 2000 Clinical Veterinary Microbiology London: Mosby;
    [Google Scholar]
  14. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  15. Wyss C., Choi B. K., Schupbach P., Guggenheim B., Göbel U. B. 1996; Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46:745–752 [CrossRef]
    [Google Scholar]
  16. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
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