1887

Abstract

A taxonomic study was performed on 13 bacterial strains isolated from preputial swabs of European bison () bulls suffering from balanoposthitis. The isolates were Gram-positive, non-motile, facultatively anaerobic, diphtheroid-shaped cells. Based on biochemical profiles and BOX-PCR-generated genomic fingerprints, the isolates were grouped into two clusters represented by four and nine strains, respectively. Strains 1(W3/01) and 2(W106/04), selected as representatives of the two clusters, shared 97·2 % 16S rRNA gene sequence similarity. The highest gene sequence similarities found (95·5–96·4 %) were to DSM 20630 and DSM 9152, demonstrating that the novel strains are members of the genus , but are not members of a recognized species. The polar lipid profiles of the two novel strains displayed the major characteristics also found in DSM 20630 and DSM 20595. Detection of a quinone system with MK-10(H) as the predominant compound confirmed phylogenetic relatedness of the novel strains to and separated them from the type species of the genus, , which contains MK-9(H) as the predominant quinone. Results from DNA–DNA hybridizations clearly demonstrated that strains 1(W3/01) and 2(W106/04) represent separate species. Based on these data, two novel species of the genus are described, for which the names sp. nov. [type strain 1(W3/01)=DSM 17162=NCTC 13354] and sp. nov. [type strain 2(W106/04)=DSM 17163=NCTC 13355] are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63923-0
2006-04-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/4/861.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63923-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Bernard, K. A., Bellefeuille, M. & Ewan, E. P. ( 1991; ). Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods. J Clin Microbiol 29, 83–89.
    [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Jones, D. & Schofield, G. M. ( 1982; ). Reclassification of ‘Corynebacterium haemolyticum’ (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen. nov. as Arcanobacterium haemolyticum nom. rev., comb. nov. J Gen Microbiol 128, 1279–1281.
    [Google Scholar]
  5. Collins, M. D., Jones, D. & Schofield, G. M. ( 1983; ). Arcanobacterium haemolyticum nom. rev., comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 10. Int J Syst Bacteriol 33, 438–440.[CrossRef]
    [Google Scholar]
  6. Jakob, W., Schröder, H. D., Rudolph, M., Krasinski, Z. A., Krasinska, M., Wolf, O., Lange, A., Cooper, J. E. & Frölich, K. ( 2000; ). Necrobacillosis in free-living male European bison in Poland. J Wildl Dis 36, 248–256.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P., Buczolits, S., Albrecht, A., Busse, H.-J. & Stackebrandt, E. ( 2003; ). Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53, 893–896.[CrossRef]
    [Google Scholar]
  9. Kita, J., Dziaba, K., Piusinski, W. & 8 other authors ( 1994; ). Preliminary studies on the diagnosis of a disease of the genital organs of male European bison in the Bialowieza Forest of Poland. IUCN Species Surviv Comm Vet Spec Group Newsl 8, 8–9.
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Louws, F. J., Fulbright, D. W., Stephens, C. T. & de Bruijn, F. J. ( 1994; ). Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 60, 2286–2295.
    [Google Scholar]
  12. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  13. Quinn, P. J., Carter, M. E., Markey, B. & Carter, G. R. ( 2000; ). Clinical Veterinary Microbiology. London: Mosby.
  14. Ventosa, A., Marquez, M. C., Kocur, M. & Tindall, B. J. ( 1993; ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. Int J Syst Bacteriol 43, 245–248.[CrossRef]
    [Google Scholar]
  15. Wyss, C., Choi, B. K., Schupbach, P., Guggenheim, B. & Göbel, U. B. ( 1996; ). Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46, 745–752.[CrossRef]
    [Google Scholar]
  16. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63923-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63923-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 861 - 866



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error