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Abstract

Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus of the ; sequence similarity values with their nearest phylogenetic neighbour, , were 97.1–98.3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA–DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related species with validly published names. The isolates were aerobic, Gram-negative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65.2 to 65.9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, sp. nov. [nine strains, with 1245 (=CIP 108725=PNCM 10346) as the type strain] and sp. nov. [one strain, the type strain 1263a (=CIP 108726=PNCM 10347)]. It is also suggested that sp. LMG 1197 (=JMP 134) should be included in the species .

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2006-05-01
2020-01-27
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vol. , part 5, pp. 973 - 978

Whole-cell protein profiles of the group P strains (representative strain 1245 ) and strain 1263 . Lanes: 1, molecular mass markers; 2, strain 1250; 3, strain 1278a; 4, strain 1246a; 5, strain 1243; 6, strain 1244a; 7, strain 1247; 8, strain 1266; 9, strain 1245 ; 10, strain 1249; 11, strain 1263 . The molecular mass markers are (from top to bottom): macroglobulin, 170000; β-galactosidase, 116400; fructose-6-phosphate kinase, 85200; glutamate dehydrogenase, 55600; aldolase, 39200; triosephosphate isomerase, 26600; trypsin inhibitor, 20100.



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