1887

Abstract

A haloalkaliphilic archaeon (strain DS12) isolated from Lake Zabuye, the Tibetan Plateau, China, was characterized to elucidate its taxonomy. The strain was aerobic, chemo-organotrophic, and grew optimally at 40 °C, pH 9·5–10·0 and 3·4 M NaCl. Cells of strain DS12 were non-motile cocci and stained Gram-variable. The major polar lipids of strain DS12 were diphytanyl and phytanyl-sesterterpanyl diether derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Phylogenetic analysis revealed that the strain formed a distinct lineage within the family . The low 16S rRNA gene sequence similarity values to its closest relatives (91·5–92·5 %) and its signature bases both suggest that the strain has no close affinity with any members of the family with validly published names. Therefore, it is proposed that strain DS12 (=AS 1.3240=JCM 11890) represents the type strain of a novel species in a new genus, gen. nov., sp. nov.

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2005-11-01
2024-12-13
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References

  1. Gibbons N. E. 1974; Halobacteriaceae .. In Bergey's Manual of Determinative Bacteriology , 8th edn. pp  269–273 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; Order I. Halobacteriales . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  294–334 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  3. Hezayen F. F., Tindall B. J., Steinbuchel A., Rehm B. H. A. 2002; Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov. and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52:2271–2280 [CrossRef]
    [Google Scholar]
  4. Kamekura M., Dyall-Smith M. L. 1995; Taxonomy of the family Halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba . J Gen Appl Microbiol 41:333–350 [CrossRef]
    [Google Scholar]
  5. Kamekura M., Dyall-Smith M. L., Upasani V., Ventosa A., Kates M. 1997; Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum , Natronobacterium magadii , and Natronobacterium pharaonis toHalorubrum , Natrialba , and Natronomonas gen. nov.,respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47:853–857 [CrossRef]
    [Google Scholar]
  6. Kanai H., Kobayashi T., Aono R., Kudo T. 1995; Natronococcus amylolyticus sp. nov., a haloalkaliphilic archaeon. Int J Syst Bacteriol 45:762–766 [CrossRef]
    [Google Scholar]
  7. McGenity T. J., Grant W. D. 1993; The haloalkaliphilic archaeon (archaebacterium) Natronococcus occultus represents a distinct lineage within the Halobacteriales , most closely related to the other haloalkaliphilic lineage ( Natronobacterium . Syst Appl Microbiol 16:239–243
    [Google Scholar]
  8. Morth S., Tindall B. J. 1985; Variation of polar lipid composition within haloalkaliphilic archaeobacteria. Syst Appl Microbiol 6:247–250 [CrossRef]
    [Google Scholar]
  9. Mwatha W. E., Grant W. D. 1993; Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int J Syst Bacteriol 43:401–404 [CrossRef]
    [Google Scholar]
  10. Oren A., Gurevich P., Gemmell R. T., Teske A. 1995; Halobaculum gomorrense gen. nov., sp. nov., a novel extremely halophilic archaeon from the Dead Sea. Int J Syst Bacteriol 45:747–754 [CrossRef]
    [Google Scholar]
  11. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  12. Oren A., Elevi R., Watanabe S., Ihara K., Corcelli A. 2002; Halomicrobium mukohataei gen. nov., comb. nov. and emended description of Halomicrobium mukohataei . Int J Syst Evol Microbiol 52:1831–1835 [CrossRef]
    [Google Scholar]
  13. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  14. Ross H. N. M., Grant W. D., Harris J. E. 1985; Lipids in archaebacterial taxonomy. In Chemical Methods in Bacterial Systematics pp  289–299 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  16. Tindall B. J., Mills A. A., Grant W. D. 1980; An alkalophilic red halophilic bacterium with a low magnesium requirement from a Kenyan soda lake. J Gen Microbiol 116:257–260
    [Google Scholar]
  17. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen. nov. and Haloferax gen. nov.. Syst Appl Microbiol 8:89–99 [CrossRef]
    [Google Scholar]
  18. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  19. Vreeland R. H., Straight S., Krammes J., Dougherty K., Rosenzweig W. D., Kamekura M. 2002; Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6:445–452 [CrossRef]
    [Google Scholar]
  20. Wainø M., Tindall B. J., Ingvorsen K. 2000; Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50:183–190 [CrossRef]
    [Google Scholar]
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