1887

International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 1

sp. nov., isolated from human faeces Free

Abstract

During studies of the microbiota of human faeces, five strains of Gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. These strains belonged to the genus on the basis of 16S rRNA gene sequence data. 16S rRNA gene sequence similarity between one of the strains, 341, and recognized species within the genus was <95 %. The DNA G+C content (44 mol%) and major fatty acid composition (anteiso-C, 32·0 %) supported the affiliation of strain 341 to the genus . Biochemical tests and DNA–DNA hybridization analysis demonstrated that strain 341 was distinct from and , to which it was related most closely. On the basis of these data, a novel species, sp. nov., is proposed with strain 341 (=JCM 13265=DSM 17393) as the type strain.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63914-0
2006-01-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/151.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63914-0&mimeType=html&fmt=ahah

References

  1. Bardocz, S., Grant, G., Brown, D. S., Ralph, A. & Pusztai, A.(1993). Polyamines in food – implications for growth and health. J Nutr Biochem 4, 66–71.[CrossRef] [Google Scholar]
  2. Benno, Y., Watabe, J. & Mitsuoka, T.(1983).Bacteroides pyogenes sp. nov., Bacteroides suis sp. nov., and Bacteroides helcogenes sp. nov., new species from abscesses and feces of pigs. Syst Appl Microbiol 4, 396–407.[CrossRef] [Google Scholar]
  3. Capano, G., Bloch, K. J., Carter, E. A., Dascoli, J. A., Schoenfeld, D. & Harmatz, P. R.(1998). Polyamines in human and rat milk influence intestinal cell growth in vitro. J Pediatr Gastroenterol Nutr 27, 281–286.[CrossRef] [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridisation in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Fenner, L., Roux, V., Mallet, M. N. & Raoult, D.(2005).Bacteroides massiliensis sp. nov., isolated from blood culture of a newborn. Int J Syst Evol Microbiol 55, 1335–1337.[CrossRef] [Google Scholar]
  6. Jousimies-Somer, H. R., Summanen, P. H., Wexler, H., Finegold, S. M., Gharbia, S. E. & Shah, H. N.(2003).Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic Gram-negative bacteria. In Manual of Clinical Microbiology, 8th edn, pp. 880–901. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  7. Kitahara, M., Takamine, F., Imamura, T. & Benno, Y.(2001).Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity. Int J Syst Evol Microbiol 51, 39–44. [Google Scholar]
  8. Kitahara, M., Sakamoto, M., Ike, M., Sakata, S. & Benno, Y.(2005).Bacteroides plebeius sp. nov. and Bacteroides coprocola sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 55, 2143–2147.[CrossRef] [Google Scholar]
  9. McCormack, S. A., Blanner, P. M., Zimmerman, B. J., Ray, R., Poppleton, H. M., Patel, T. B. & Johnson, L. R.(1998). Polyamine deficiency alters EGF receptor distribution and signaling effectiveness in IEC-6 cells. Am J Physiol 274, C192–C205. [Google Scholar]
  10. Miyagawa, E., Azuma, R. & Suto, T.(1979). Cellular fatty acid composition in Gram-negative obligately anaerobic rods. J Gen Appl Microbiol 25, 41–51.[CrossRef] [Google Scholar]
  11. Noack, J., Kleessen, B., Proll, J., Dongowski, G. & Blaut, M.(1998). Dietary guar gum and pectin stimulate intestinal microbial polyamine synthesis in rats. J Nutr 128, 1385–1391. [Google Scholar]
  12. Noack, J., Dongowski, G., Hartmann, L. & Blaut, M.(2000). The human gut bacteria Bacteroides thetaiotaomicron and Fusobacterium varium produce putrescine and spermidine in cecum of pectin-fed gnotobiotic rats. J Nutr 130, 1225–1231. [Google Scholar]
  13. Saito, H. & Miura, K.(1963). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sakamoto, M., Suzuki, M., Umeda, M., Ishikawa, I. & Benno, Y.(2002). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52, 841–849.[CrossRef] [Google Scholar]
  16. Satink, H. P. W. M., Hessels, J., Kingma, A. W., van den Berg, G. A., Muskiet, F. A. J. & Halie, M. R.(1989). Microbial influences on urinary polyamine excretion. Clin Chim Acta 179, 305–314.[CrossRef] [Google Scholar]
  17. Shah, H. N.(1992). The genus Bacteroides and related taxa. In The Prokaryotes, 2nd edn, pp. 3593–3607. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  18. Song, Y. L., Liu, C. X., McTeague, M. & Finegold, S. M.(2004). “Bacteroides nordii” sp. nov. and “Bacteroides salyersae” sp. nov. isolated from clinical specimens of human intestinal origin. J Clin Microbiol 42, 5565–5570.[CrossRef] [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63914-0
Loading
Bacteroides intestinalis sp. nov., isolated from human faeces
Int J Syst Evol Microbiol 56, 151 (2006); https://doi.org/10.1099/ijs.0.63914-0
/content/journal/ijsem/10.1099/ijs.0.63914-0
/content/journal/ijsem/10.1099/ijs.0.63914-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 151 - 154

API 20 A and API rapid ID 32 A test results.

Cellular fatty acid compositions of sp. nov., and .

DNA base composition and levels of DNA–DNA hybridization among the novel species and related strains.

[PDF file of Supplementary Tables S1-S3](29 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error