@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63912-0, author = "Wartiainen, Ingvild and Hestnes, Anne Grethe and McDonald, Ian R. and Svenning, Mette M.", title = "Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78° N)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2006", volume = "56", number = "3", pages = "541-547", doi = "https://doi.org/10.1099/ijs.0.63912-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63912-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "PHB, poly-β-hydroxybutyrate", abstract = "A Gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, SV97T, was isolated from a wetland soil near Ny-Ålesund, Svalbard Islands, Norway (78° N). On the basis of 16S rRNA gene sequence similarity, strain SV97T was shown to belong to the Alphaproteobacteria and was highly related to a number of non-characterized Methylocystis strains with GenBank accession nos AJ458507 and AJ458502 (100 %) and AF177299, AJ458510, AJ458467, AJ458471, AJ431384, AJ458475, AJ458484, AJ458501 and AJ458466 (99 %). The most closely related type strains were Methylocystis parvus OBBPT (97·2 %) and Methylocystis echinoides IMET 10491T (97 %). The closest related recognized species within the genus Methylosinus was Methylosinus sporium NCIMB 11126T (96·0 % similarity). Chemotaxonomic and phenotypic data (C18 : 1 ω8 as the major fatty acid, non-motile, no rosette formation) supported the affiliation of strain SV97T to the genus Methylocystis. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV97T from the two recognized Methylocystis species. Strain SV97T therefore represents a novel species, for which the name Methylocystis rosea sp. nov. is proposed, with the type strain SV97T (=DSM 17261T=ATCC BAA-1196T).", }