Leuconostoc argentinum, Leuconostoc lactis and ten related strains from Romanian dairy products formed a single cluster, clearly separated from other Leuconostoc species, after numerical analysis of repetitive extragenic palindromic-PCR patterns, whole-cell protein profiles (SDS-PAGE) and fluorescent amplified fragment length polymorphism (FAFLP) band patterns. 16S rRNA gene sequence analysis confirmed a very high similarity between both type strains and representative dairy isolates (>99·6 %). DNA–DNA hybridization experiments revealed high relatedness values between the type strains of L. argentinum and L. lactis and between these strains and representative Romanian strains. These data and the lack of phenotypic distinctive characteristics demonstrate that L. argentinum and L. lactis are synonymous.
de Man, J. C., Rogosa, M. & Sharpe, M. E.(1960). A medium for the cultivation of lactobacilli. J Appl Bacteriol23, 130–135.[CrossRef][Google Scholar]
Dicks, L. M. T., Fantuzzi, L., Gonzalez, F. C., Du Toit, M. & Dellaglio, F.(1993).Leuconostoc argentinum sp. nov., isolated from Argentine raw milk. Int J Syst Bacteriol43, 347–351.[CrossRef][Google Scholar]
Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39, 224–229.[CrossRef][Google Scholar]
Farrow, J. A. E., Facklam, R. R. & Collins, M. D.(1989). Nucleic acid homologies of some vancomycin-resistant leuconostocs and description of Leuconostoc citreum sp. nov. and Leuconostoc pseudomesenteroides sp. nov. Int J Syst Bacteriol39, 279–283.[CrossRef][Google Scholar]
Gancheva, A., Pot, B., Vanhonacker, K., Hoste, B. & Kersters, K.(1999). A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species. Syst Appl Microbiol22, 573–585.[CrossRef][Google Scholar]
Garvie, E. I.(1960). The genus Leuconostoc and its nomenclature. J Dairy Res27, 283–292.[CrossRef][Google Scholar]
Garvie, E. I.(1986). Genus Leuconostoc. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1071–1075. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
Gevers, D., Huys, G. & Swings, J.(2001). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett205, 31–36.[CrossRef][Google Scholar]
Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K.(1998). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol44, 1148–1153.[CrossRef][Google Scholar]
Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol3, 208–218.[CrossRef][Google Scholar]
Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39, 159–167.[CrossRef][Google Scholar]
Pot, B., Vandamme, P. & Kersters, K.(1994). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J.(2001). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol24, 520–538.[CrossRef][Google Scholar]
Torriani, S., Clementi, F., Vancanneyt, M., Hoste, B., Dellaglio, F. & Kersters, K.(2001). Differentiation of Lactobacillus plantarum, L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP. Syst Appl Microbiol24, 554–560.[CrossRef][Google Scholar]
Vancanneyt, M., Mengaud, J., Cleenwerck, I., Hoste, B. & 7 other authors(2004). Reclassification of Lactobacillus kefirgranum Takizawa et al. 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp. nov. and emended description of L. kefiranofaciens Fujisawa et al. 1988. Int J Syst Evol Microbiol54, 551–556.[CrossRef][Google Scholar]
International Journal of Systematic and Evolutionary
Microbiology vol.
56 , part 1, pp. 213 - 216
Supplementary Fig. S1. A dendrogram based on (GTG)
5-PCR fingerprints and
Supplementary Table S1. Phenotypic features of
Leuconostoc lactis strains.
[PDF](137 KB)