1887

Abstract

A novel actinomycete, strain N3-7, was isolated from a natural cave in Jeju, Republic of Korea, using a dilution method and was subjected to characterization using polyphasic taxonomy. A 16S rRNA gene sequence analysis revealed that the organism belonged to the phylogenetic cluster of the genus and was most closely related to and (97.6 and 97.5 % similarity, respectively). The main chemotaxonomic properties of strain N3-7, such as the principal amino acid of the peptidoglycan, the predominant menaquinone and the polar lipid profile, supported classification in the genus . The organism was readily differentiated from species with validly published names on the basis of a broad range of phenotypic properties. Thus the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain N3-7 (=JCM 13278=NRRL B-24429).

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2006-05-01
2019-10-21
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References

  1. Brosius, J., Palmer, M. L., Kennedy, J. P. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  4. Hopwood, D. A., Bibb, M. J., Chater, K. F. & 7 other authors ( 1985; ). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation.
  5. Iinuma, S., Yokota, A., Hasegawa, T. & Kanamaru, T. ( 1994; ). Actinocorallia gen. nov., a new genus of the order Actinomycetales. Int J Syst Bacteriol 44, 230–234.[CrossRef]
    [Google Scholar]
  6. Itoh, T., Kudo, T., Oyaizu, H. & Seino, A. ( 1995; ). Two new species in the genus Actinomadura: A. glomerata sp. nov., and A. longicatena sp. nov. Actinomycetology 9, 164–177.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  9. Kroppenstedt, R. M., Stackebrandt, E. & Goodfellow, M. ( 1990; ). Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora. Syst Appl Microbiol 13, 148–160.[CrossRef]
    [Google Scholar]
  10. Lavrova, N. V. & Preobrazhenskaya, T. P. ( 1975; ). Isolation of new species of the genus Actinomadura on selective media with rubomycin. Antibiotiki 20, 483–488 (in Russian).
    [Google Scholar]
  11. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Composition of whole cell hydrolysates as a criterion in the classification of aerobic actinomycetes. In The Actinomycetales, pp. 311–316. Edited by H. Prauser. Jena: Gustav Fischer.
  12. Lee, S. D., Kang, S.-O. & Hah, Y. C. ( 2000a; ). Hongia gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol 50, 191–199.[CrossRef]
    [Google Scholar]
  13. Lee, S. D., Kang, S.-O. & Hah, Y. C. ( 2000b; ). Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave. Int J Syst Evol Microbiol 50, 1103–1111.[CrossRef]
    [Google Scholar]
  14. MacFaddin, J. F. ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Meyer, J. ( 1979; ). New species of the genus Actinomadura. Z Allg Mikrobiol 19, 37–44.[CrossRef]
    [Google Scholar]
  17. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1977; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.
    [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  19. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, E. & Selva, E. ( 1991; ). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24, 4876–4882.
    [Google Scholar]
  24. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  25. Zhang, Z. S., Wang, Y. & Ruan, J. S. ( 1998; ). Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol 48, 411–422.[CrossRef]
    [Google Scholar]
  26. Zhang, Z., Kudo, T., Nakajima, Y. & Wang, Y. ( 2001; ). Clarification of the relationship between the members of the family Thermomonosporaceae on the basis of the 16S rDNA, 16S–23S rRNA internal transcribed spacer and 23S rDNA sequences and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 373–383.
    [Google Scholar]
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vol. , part 5, pp. 1085-1088

A complete neighbour-joining tree showing the relationships between strain N3-7 and representatives of the genus . [PDF](22 KB)



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