1887

Abstract

In order to clarify the intraspecies taxonomic position of the non-photosynthetic algal species , as well as the aetiology of bovine mammary protothecosis, a selection of strains isolated from clinical cases of protothecal mastitis and from various environmental habitats was characterized using a polyphasic molecular approach. Based on sequence analysis of the 18S rRNA gene, which showed distinct differences between the three currently known biotypes or ‘variants’, specific oligonucleotides were designed and used in biotype-specific PCRs. Furthermore, the pattern of cellular fatty acids was evaluated. Typing by means of these techniques revealed that the previously defined biotypes of were clearly different. Based on sequence analysis, the pattern of fatty acids and physiological characteristics, it is proposed that biotype 3 should be reclassified as representing a novel species, sp. nov. (type strain, RZIII-3=SAG 2064). Furthermore, it is proposed that merits reclassification as a species comprising at least two genotypes that in future could probably be considered to represent two subspecies.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63892-0
2006-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/6/1419.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63892-0&mimeType=html&fmt=ahah

References

  1. Arnold P., Ahearn D. G. 1972; The systematics of the genus Prototheca with a description of a new species P. filamenta . Mycologia 64:265–275 [CrossRef]
    [Google Scholar]
  2. Biedermann W., Lücker E., Pörschmann J., Lachhab S., Truyen U., Hensel A. 2004; Structural characterisation of some fatty acids from the brain as biomarkers of BSE risk material. Anal Bioanal Chem 379:1031–1038
    [Google Scholar]
  3. Blaschke-Hellmessen R., Schuster H., Bergmann V. 1985; Differenzierung von Varianten bei Prototheca zopfii (Krüger 1894. Arch Exp Veterinarmed 39:387–397 (in German
    [Google Scholar]
  4. Blaschke-Hellmessen R., Wilhelm A., Teichmann G., Schuster H., Boeltzig K. 1987; Orientierende Untersuchungen zum Nachweis von Antikörpern gegen Prototheca zopfii bei Rindern. Monatsh Veterinarmed 42:48–50 (in German
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Jobb G., von Haeseler A., Strimmer K. 2004; treefinder: a powerful graphical analysis environment for molecular phylogenetics. BMC Evol Biol 4:18 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Krüger W. 1894; Kurze Charakteristik einiger niederer Organismen im Saftflusse der Laubbäume. Hedwigia XXXIII:16–241 (in German
    [Google Scholar]
  9. Pore R. S. 1985; Prototheca taxonomy. Mycopathologia 90:129–139 [CrossRef]
    [Google Scholar]
  10. Pore R. S. 1986; The association of Prototheca spp. with slime flux in Ulmus amencana and other trees. Mycopathologia 94:67–73 [CrossRef]
    [Google Scholar]
  11. Roesler U., Scholz H., Hensel A. 2001; Immunodiagnostic identification of dairy cows infected with Prototheca zopfii at various clinical stages and discrimination between infected and uninfected cows. J Clin Microbiol 39:539–543 [CrossRef]
    [Google Scholar]
  12. Roesler U., Scholz H., Hensel A. 2003; Emended phenotypic characterization of Prototheca zopfii : a proposal for three biotypes and standards for their identification. Int J Syst Evol Microbiol 53:1195–1199 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Schmalreck A. F., Trankle P., Vanca E., Blaschke-Hellmessen R. 1998; Differenzierung und Charakterisierung von humanpathogenen Hefen ( Candida albicans , Exophiala dermatidis ) und tierpathogenen Algen ( Prototheca spp.) mittels Fourier-Transform-Infrarot-Spektroskopie (FT-IR) im Vergleich zu konventionellen Methoden. Mycoses 41 (Suppl. 1):71–77 (in German [CrossRef]
    [Google Scholar]
  15. Schuster H., Blaschke-Hellmessen R. 1983; Zur Epidemiologie der Protothekenmastitis des Rindes – Anzüchtung von Algen der Gattung Prototheca aus der Umgebung landwirtschaftlicher Nutztiere. Monatsh Veterinarmed 38:24–29 (in German
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  17. Ueno R., Urano N., Suzuki M. 2003; Phylogeny of the non-photosynthetic green micro-algal genus Prototheca (Trebouxiophyceae, Chlorophyta) and related taxa inferred from SSU and LSU ribosomal DNA partial sequence data. FEMS Microbiol Lett 223:275–280 [CrossRef]
    [Google Scholar]
  18. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63892-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63892-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error