Two methanogenic strains, 8Ac and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8Ac and 6Ac were rod-shaped (0·8–1·0×3–5 μm) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8Ac was 0·030 h when growing in acetate (20 mM) at 37 °C. The temperature range for growth was 25–45 °C, with the fastest growth at 34–37 °C. The pH range for growth and methane production was 6·5–9·0, with the fastest growth at pH 7·2–7·6. The G+C content of genomic DNA of strain 8Ac was 55·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with species; the 16S rRNA gene sequence similarities between strain 8Ac and DSM 3013 and ‘’ DSM 6194 were 92·5 and 87·3 %, respectively. The sequence similarity levels of , the gene encoding the -subunit of methyl-coenzyme M reductase, and of the deduced amino acids of , between strain 8Ac and DSM 3671 were 36 and 78·9 %, respectively. Based on the phylogenetic and phenotypic analyses, the novel species sp. nov. is proposed, with strain 8Ac (=JCM 13211=CGMCC 1.5026) as the type strain.


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  1. Boone, D. R. & Kamagata, Y.(1998). Rejection of the species Methanothrix soehngeniiVP and the genus MethanothrixVP as nomina confusa, and transfer of Methanothrix thermophilaVP to the genus MethanosaetaVP as Methanosaeta thermophila comb. nov. Request for an Opinion. Int J Syst Bacteriol 48, 1079–1080.[CrossRef] [Google Scholar]
  2. Boone, D. R. & Whitman, W. B.(1988). Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int J Syst Bacteriol 38, 212–219.[CrossRef] [Google Scholar]
  3. Furlong, M. A., Singleton, D. R., Coleman, D. C. & Whitman, W. B.(2002). Molecular and culture-based analyses of prokaryotic communities from an agricultural soil and the burrows and casts of the earthworm Lumbricus rubellus. Appl Environ Microbiol 68, 1265–1279.[CrossRef] [Google Scholar]
  4. Gonzalez-Gil, G., Lens, P. N. L., Van Aelst, A., Van As, H., Versprille, A. I. & Lettinga, G.(2001). Cluster structure of anaerobic aggregates of an expanded granular sludge bed reactor. Appl Environ Microbiol 67, 3683–3692.[CrossRef] [Google Scholar]
  5. Guiot, S. R., Pauss, A. & Costerton, J. W.(1992). A structured model of the anaerobic granule consortium. Water Sci Technol 25 (7–8), 1–10. [Google Scholar]
  6. Huser, B. A., Wuhrmann, K. & Zehnder, A. J. B.(1982).Methanothrix soehngenii gen. nov. sp. nov., a new acetotrophic non-hydrogen-oxidizing methane bacterium. Arch Microbiol 132, 1–9.[CrossRef] [Google Scholar]
  7. Jarrell, K. F., Faguy, D., Hebert, A. M. & Kalmokoff, M. L.(1992). A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria). Can J Microbiol 38, 65–68.[CrossRef] [Google Scholar]
  8. Kamagata, Y., Kawasaki, H., Oyaizu, H., Nakamura, K., Mikami, E., Endo, G., Koga, Y. & Yamasato, K.(1992). Characterization of three thermophilic strains of Methanothrix (“Methanosaeta”) thermophila sp. nov. and rejection of Methanothrix (“Methanosaeta”) thermoacetophila. Int J Syst Bacteriol 42, 463–468.[CrossRef] [Google Scholar]
  9. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: Molecular Evolutionary Genetics Analysis software. Arizona State University, Tempe, AZ, USA.
  10. Lueders, T., Chin, K. J., Conrad, R. & Friedrich, M.(2001). Molecular analyses of methyl-coenzyme M reductase α-subunit (mcrA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage. Environ Microbiol 3, 194–204.[CrossRef] [Google Scholar]
  11. Ma, K., Liu, X. & Dong, X.(2005).Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters. Int J Syst Evol Microbiol 55, 325–329.[CrossRef] [Google Scholar]
  12. MacLeod, F. A., Guiot, S. R. & Costerton, J. W.(1990). Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl Environ Microbiol 56, 1598–1607. [Google Scholar]
  13. Owen, R. J. & Pitcher, D.(1985). Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. In Chemical Methods in Bacterial Systematics, pp. 67–93. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  14. Patel, G. B. & Sprott, G. D.(1990).Methanosaeta concilii gen. nov., sp. nov. (“Methanothrix concilii”) and Methanosaeta thermoacetophila nom. rev., comb. nov. Int J Syst Bacteriol 40, 79–82.[CrossRef] [Google Scholar]
  15. Springer, E., Sachs, M. S., Woese, C. R. & Boone, D. R.(1995). Partial gene sequences for the a subunit of methyl-coenzyme M reductase (mcrI) as a phylogenetic tool for the family Methanosarcinaceae. Int J Syst Bacteriol 45, 554–559.[CrossRef] [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  17. Touzel, J. P., Prensier, G., Roustan, J. L., Thomas, I., Dubourguier, H. C. & Albagnac, G.(1988). Description of a new strain of Methanothrix soehngenii and rejection of Methanothrix concilii as a synonym of Methanothrix soehngenii. Int J Syst Bacteriol 38, 30–36.[CrossRef] [Google Scholar]
  18. Zengler, K., Richnow, H. H., Rosselló-Mora, R., Michaelis, W. & Widdel, F.(1999). Methane formation from long-chain alkanes by anaerobic microorganisms. Nature 401, 266–269.[CrossRef] [Google Scholar]
  19. Zhang, C., Liu, X. & Dong, X.(2004).Syntrophomonas curvata sp. nov., an anaerobe that degrades fatty acids in co-culture with methanogens. Int J Syst Evol Microbiol 54, 969–973.[CrossRef] [Google Scholar]
  20. Zinder, S. H.(1993). Physiological ecology of methanogens. In Methanogenesis: Ecology, Physiology, Biochemistry and Genetics, pp. 128–206. Edited by J. G. Ferry. New York: Chapman & Hall.

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