1887

Abstract

Actinomycete strain H9 was isolated from coastal sediment of the Jiao-Dong peninsula (near Tsingdao city) in China, and was identified by means of polyphasic taxonomy. The strain grew autotrophically on modified nitrifying medium and heterotrophically on Luria–Bertani medium, with NaCl ranging from 0 to 8 % (w/v) (optimal growth at 3·5 %). The 16S rRNA gene sequence similarities of strain H9 to members of the genus ranged from 93·0 to 97·5 %, indicating that strain H9 was phylogenetically related to members of the genus . Strain H9 had type IV cell wall and type PIII phospholipid, and its major menaquinone was MK-8 (H). DNA–DNA relatedness values between strain H9 and , and were 42, 13 and 11 %, respectively. These results support the conclusion that H9 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain H9 (=CGMCC 4.1877=JCM 12462).

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2006-03-01
2024-03-29
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References

  1. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation;
    [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Norcardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  5. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  6. Henssen A. 1957; Beiträge zur morphologie und systematik der thermophilen actinomyceten. Arch Mikrobiol 26:373–414 (in German [CrossRef]
    [Google Scholar]
  7. Hu Y.-T., Zhou P.-J., Zhou Y.-G., Liu Z.-H., Liu S.-J. 2004; Saccharothrix xingjiangensis , sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjiang, China. Int J Syst Evol Microbiol 54:2091–2094 [CrossRef]
    [Google Scholar]
  8. Huang Y., Wang L., Lu Z., Hong L., Liu Z., Tan G. Y. A., Goodfellow M. 2002; Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia , and description of Pseudonocardia zijingensis sp. nov. Int J Syst Evol Microbiol 52:977–982 [CrossRef]
    [Google Scholar]
  9. Jahnke K.-D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. 2004; Pseudonocardia benzenivorans sp. nov. Int J Syst Evol Microbiol 54:749–751 [CrossRef]
    [Google Scholar]
  11. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  12. Lechevalier H. A., Lechevalier M. P. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy , Special Publication no. 6 pp  277–284 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  13. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  14. Lee S. D., Kim E. S., Min K.-L., Lee W. Y., Kang S.-O., Hah Y. C. 2001; Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave. Int J Syst Evol Microbiol 51:1505–1510
    [Google Scholar]
  15. Lee S.-B., Strand S. E., Stensel H. D., Herwig R. P. 2004; Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete. Int J Syst Evol Microbiol 54:131–139 [CrossRef]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  17. McVeigh H. P., Munro J., Embley T. M. 1994; The phylogenetic position of Pseudoamycolata halophobica (Akimov et al . 1989) and a proposal to reclassify it as Pseudonocardia halophobica . Int J Syst Bacteriol 44:300–302 [CrossRef]
    [Google Scholar]
  18. Prabahar V., Dube S., Reddy G. S. N., Shivaji S. 2004; Pseudonocardia antarctica sp. nov., an Actinomycetes from McMurdo Dry Valleys, Antarctica. Syst Appl Microbiol 27:66–71 [CrossRef]
    [Google Scholar]
  19. Rand M. C., Greenberg A. E., Taras J. M. (editors) 1975; Standard Methods for the Examination of Water and Wastewater . , 14th edn. pp  434–436 Washington, DC: American Public Health Association;
  20. Reichert K., Lipski A., Pradella S., Stackebrandt E., Altendorf K. 1998; Pseudonocardia asacccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia . Int J Syst Bacteriol 48:441–449 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Slawyk G., Maclsaac J. J. 1972; Comparison of two automated ammonium methods in a region of coastal upwelling. Deep Sea Res 19:521–524
    [Google Scholar]
  23. Takamiya A., Tubaki K. 1956; A new form of Streptomyces capable of growing autotrophically. Arch Mikrobiol 25:58–64 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  25. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  26. van't Riet J., Stouthamer A. H., Planta R. J. 1968; Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes . J Bacteriol 96:1455–1464
    [Google Scholar]
  27. Warwick S., Bowen T., McVeigh H. P., Embley T. M. 1994; A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia . Int J Syst Bacteriol 44:293–299 [CrossRef]
    [Google Scholar]
  28. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology (English translation of Mikrobiologiia) 16:176–178
    [Google Scholar]
  29. Zhang D., Yang H., Zhang W., Huang Z., Liu S.-J. 2003; Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53:1111–1114 [CrossRef]
    [Google Scholar]
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