1887

Abstract

Six strains with more than 99·5 % 16S rRNA gene sequence similarity, identical internal spacer region profiles and restriction analysis of the amplified 16S rRNA gene patterns were isolated from fermenting grape musts during independent studies carried out in France and Spain many years apart. Strains are Gram-positive, motile, facultatively anaerobic rods that do not exhibit catalase activity and have the ability to utilize pentose sugars (ribose and/or -arabinose), although they are homofermentative bacteria. Strains ferment pentoses exclusively yielding lactic acid as the end product. A broad set of molecular techniques has been applied to characterize these strains and the results show a high degree of genotypical congruence, sharing identical profiles with 16S rRNA-based techniques. Phylogenetic analysis based on 16S rRNA gene sequences placed these strains within the genus , closely related to , and (with approximately 95 % sequence similarity). DNA–DNA hybridization experiments confirmed the independent status at the species level of these fermenting grape-musts strains. Phenotypically they can be distinguished from the closest relatives by several traits such as growth temperatures and fermentation of carbohydrates. The name sp. nov. is proposed, with strain Mont 4 (=DSM 20605=CECT 5924) as the type strain.

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2006-03-01
2019-12-06
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vol. , part 3, pp. 513 - 517

Phenotypic characteristics of sp. nov. and its closest phylogenetic neighbours , and

Phylogenetic trees constructed using the maximum-parsimony and maximum-likelihood methods

Dendrogram derived from comparison of the combined molecular techniques’ patterns obtained from wine lactic acid bacteria and reference strains [Single PDF file](655 KB)



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