1887

Abstract

Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic, coccus-shaped bacterium, originating from a porpoise and a grey seal, were characterized using phenotypic, biochemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on -lysine (type -lys–gly–-Asp) and a DNA G+C content of 38 mol%. Comparative 16S rRNA gene sequencing showed that this unidentified coccus-shaped organism formed a hitherto unknown subline closely related to, albeit distinct from, a number of other catalase-negative genera which included , , and . Other known Gram-positive, catalase-negative taxa were more distantly related. Tree-branching considerations and sequence divergence values of >6 % with recognized taxa were indicative of this novel bacterium representing a separate genus. Based on phenotypic and phylogenetic evidence, it is proposed that this unknown bacterium, recovered from a porpoise and a grey seal, be classified as a novel genus and species, gen. nov., sp. nov. The type strain is M35/04/3 (=CCUG 49459=CIP 108575).

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2006-02-01
2019-08-25
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References

  1. Aguirre, M. & Collins, M. D. ( 1992; ). Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid. Int J Syst Bacteriol 42, 79–83.[CrossRef]
    [Google Scholar]
  2. Aguirre, M., Morrison, D., Cookson, B. D., Gay, F. W. & Collins, M. D. ( 1993; ). Phenotypic and phylogenetic characterisation of some Gemella-like organisms from human infections: description of Dolosigranulum pigrum gen. nov., sp. nov. J Appl Bacteriol 75, 608–612.[CrossRef]
    [Google Scholar]
  3. Bailey, L. & Collins, M. D. ( 1982; ). Reclassification of Streptococcus pluton (White) in a new genus Melissococcus, as Melissococcus pluton nom. rev.; comb. nov. J Appl Bacteriol 53, 215–217.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Ash, C., Farrow, J. A. E., Wallbanks, S. & Williams, A. M. ( 1989; ). 16S Ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. J Appl Bacteriol 67, 453–460.[CrossRef]
    [Google Scholar]
  5. Collins, M. D., Aguirre, M., Facklam, R. R., Shallcross, J. & Williams, A. M. ( 1992; ). Globicatella sanguis gen. nov., sp. nov., a new gram-positive catalase. negative bacterium from human sources. J Appl Bacteriol 73, 433–437.[CrossRef]
    [Google Scholar]
  6. Collins, M. D., Falsen, E., Lemozy, J., Åkervall, E., Sjödén, B. & Lawson, P. A. ( 1997; ). Phenotypic and phylogenetic characterization of some Globicatella-like organisms from human sources: description of Facklamia hominis gen. nov., sp. nov. Int J Syst Bacteriol 47, 880–882.[CrossRef]
    [Google Scholar]
  7. Collins, M. D., Lawson, P. A., Monasterio, R., Falsen, E., Sjödén, B. & Facklam, R. R. ( 1999; ). Ignavigranum ruoffiae sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 49, 97–101.[CrossRef]
    [Google Scholar]
  8. Facklam, R. R. ( 2002; ). What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15, 613–630.[CrossRef]
    [Google Scholar]
  9. Facklam, R. R. & Elliott, J. A. ( 1995; ). Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8, 479–495.
    [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  11. Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II ( 1997; ). GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4, 14.
    [Google Scholar]
  12. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  13. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  14. Rasmussen, S. W. ( 2002; ). SEQtools, a win32 software suite for analysis and handling of nucleotide and protein sequences. http://www.seqtools.dk
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  17. Schleifer, K. H. & Kilpper-Bälz, R. ( 1984; ). Transfer of Streptococcus faecalis and Steptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Bacteriol 34, 31–34.[CrossRef]
    [Google Scholar]
  18. Schleifer, K. H., Kraus, J., Dvorak, C., Kilpper-Balz, R., Collins, M. D. & Fischer, W. ( 1985; ). Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov. Syst Appl Microbiol 6, 183–195.[CrossRef]
    [Google Scholar]
  19. Vandamme, P., Torck, U., Falsen, E., Pot, B., Goossens, H. & Kersters, K. ( 1998; ). Whole-cell protein electrophoretic analysis of viridans Streptococcus mitis biovars. Int J Syst Bacteriol 48, 117–125.[CrossRef]
    [Google Scholar]
  20. Wallbanks, S., Martinez-Murcia, A. J., Fryer, J. L., Philips, B. A. & Collins, M. D. ( 1990; ). 16S rRNA sequence determination for members of the genus Carnobacterium and related lactic acid bacteria and description of Vagococcus salmoninarum sp. nov. Int J Syst Bacteriol 40, 224–230.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 429 - 432

Unrooted neighbour-joining tree based on 16S rRNA gene sequences including an extended sample of sequences showing the phylogenetic relationships of gen. nov., sp. nov. with other Gram-positive, catalase-negative taxa. [PDF](111 KB)



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