1887

Abstract

Three strains of a spore-forming, Gram-positive, motile, rod-shaped and boron-tolerant bacterium were isolated from soil. The strains, designated 10a, 11c and 12B, can tolerate 5 % (w/v) NaCl and up to 150 mM boron, but optimal growth was observed without addition of boron or NaCl in Luria–Bertani agar medium. The optimum temperature for growth was 37 °C (range 16–45 °C) and the optimum pH was 7.0–8.0 (range pH 5.5–9.5). A comparative analysis of the 16S rRNA gene sequence demonstrated that the isolated strains were closely related to DSM 2898 (97.2 % similarity) and DSM 28 (96.9 %). DNA–DNA relatedness was greater than 97 % among the isolated strains and 61.1 % with DSM 2898 and 43.2 % with IAM 13420. The phylogenetic and phenotypic analyses and DNA–DNA relatedness indicated that the three strains belong to the same species, that was characterized by a DNA G+C content of 36.5–37.9 mol%, MK-7 as the predominant menaquinone system and iso-C (32 % of the total) as a major cellular fatty acid. In contrast to the type species of the genus , the strains contained peptidoglycan with lysine, aspartic acid, alanine and glutamic acid. Based on the distinctive peptidoglycan composition, phylogenetic analyses and physiology, the strains are assigned to a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain 10a (=DSM 17140=IAM 15262=ATCC BAA-1146). It is also proposed that and be transferred to this genus as comb. nov. and comb. nov., respectively.

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2007-05-01
2024-03-28
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