1887

Abstract

A novel actinomycete, strain N3-2, was isolated from a natural cave on Jeju Island, Republic of Korea, using a dilution method and was subjected to polyphasic taxonomy. The almost complete 16S rRNA gene sequence was determined by direct sequencing of the purified PCR product and was compared with those of representatives of the genus . It was revealed from the phylogenetic analysis that the organism forms a distinct clade between the cluster and the branch within the evolutionary radius occupied by the genus of the family . The organism showed 16S rRNA gene sequence similarity of 97·4 % with its nearest phylogenetic neighbours, namely and . The chemotaxonomic properties, such as the principal amino acid of peptidoglycan, predominant menaquinone and polar lipids, supported the classification in the genus . The organism was readily differentiated from species with validly published names by a broad set of phenotypic properties and its unique phylogenetic position; the name sp. nov. is proposed, with N3-2 (=JCM 13281=NRRL B-24430) as the type strain.

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2006-03-01
2020-11-27
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