1887

Abstract

A Gram-negative, microaerophilic helical rod, isolated from the gastric mucosa of a dog and designated strain JKM4, was subjected to a polyphasic taxonomic study. The tightly coiled organism, measuring 10–18 μm long and up to 1 μm wide, was motile by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril running along the external side of the helix. Strain JKM4 grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 30 and 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus and showed > 97 % similarity to , and , three species previously isolated from the canine gastric mucosa. Protein profiling of strain JKM4 using SDS-PAGE revealed a pattern different from those of other species of mammalian gastric origin and from . Additionally, the urease gene sequence of strain JKM4 was different from those of urease genes of , , and ‘ Helicobacter heilmannii’. It is thus proposed that strain JKM4 (=LMG 23188) represents a novel species within this genus, sp. nov.

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2006-07-01
2019-10-14
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A phylogenetic tree based on 16S rRNA gene sequences for 27 strains and TEM images of strain JKM4 . [PDF](716 KB)

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16S rRNA gene sequence similarity between strains. [Excel](33 KB)

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