gen. nov., sp. nov., a novel, mycelium-forming actinomycete, and proposal of fam. nov. Free

Abstract

A novel, Gram-positive bacterial strain was isolated from forest soil. Among species with validly published names, the 16S rRNA gene sequence is related most closely (approx. 93 % similarity) to that of DSM 43042. However, differently from this species, it forms both vegetative and aerial mycelia. The aerial hyphae are straight to slightly flexuous, starting to septate to form chains of more than 20 cylindrical spores with a rugose surface. The strain is acidophilic, with a pH range for robust growth between 4.3 and 6.8 and an optimum around 6.0. The peptidoglycan type is A3 -Dpm–Gly. The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. Predominant menaquinones are MK-9(H) and -9(H), and iso- and anteiso-branched C and C are the main cellular fatty acids. The DNA G+C content is 71.9 mol%. The distinct phylogenetic position and the unusual combination of chemotaxonomic characteristics justify the proposal of gen. nov., with the type species sp. nov. (type strain, ID139908 =DSM 44928=NRRL B-24433). fam. nov. is also proposed.

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2006-08-01
2024-03-28
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References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Bull A. T., Ward A. C., Goodfellow M. 2000; Search and discovery strategies for biotechnology: the paradigm shift. Microbiol Mol Biol Rev 64:573–606 [CrossRef]
    [Google Scholar]
  3. Demain A. L. 1998; Microbial natural products: alive and well in 1998. Nat Biotechnol 16:3–4
    [Google Scholar]
  4. Donadio S., Monciardini P., Alduina R., Mazza P., Chiocchini C., Cavaletti L., Sosio M., Puglia A. M. 2002; Microbial technologies for the discovery of novel bioactive metabolites. J Biotechnol 99:187–198 [CrossRef]
    [Google Scholar]
  5. Goodfellow M. 1989; Suprageneric classification of actinomycetes. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2333–2339 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Gottlieb D. 1961; An evaluation of criteria and procedures used in the description and characterization of the streptomycetes. Appl Microbiol 9:55–65
    [Google Scholar]
  7. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Bogoriella caseilytica gen. nov., sp. nov. a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol 47:788–794 [CrossRef]
    [Google Scholar]
  8. Hammerschmidt S., Wolff S., Hocke A., Rosseau S., Müller E., Rohde M. 2005; Illustration of pneumococcal polysaccharide capsule during adherence and invasion of epithelial cells. Infect Immun 73:4653–4667 [CrossRef]
    [Google Scholar]
  9. Joseph S. J., Hugenholtz P., Sangwan P., Osborne C. A., Janssen P. H. 2003; Laboratory cultivation of widespread and previously uncultured soil bacteria. Appl Environ Microbiol 69:7210–7215 [CrossRef]
    [Google Scholar]
  10. Kim S. B., Lonsdale J., Seong C.-N., Goodfellow M. et al. 2003; Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici (1943)AL) emend. Rainey et al 1997. Antonie van Leeuwenhoek 83:107–116 [CrossRef]
    [Google Scholar]
  11. Korn-Wendisch F., Kutzner H. J. 1992; The family Streptomycetaceae . In The Prokaryotes , 2nd edn. vol 1 pp  921–995 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. Heidelberg: Springer;
    [Google Scholar]
  12. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  13. Lechevalier M. P., Lechevalier H. A. 1989; Genus Sporichthya Lechevalier, Lechevalier and Holbert 1968, 279AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2507–2508 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  14. Luedemann G. M., Fonseca A. F. 1989; Genus Geodermatophilus Luedemann 1968, 1857AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2406–2409 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Maerz A., Paul M. R. 1950 Dictionary of Color , 2nd edn. New York: McGraw-Hill;
    [Google Scholar]
  16. Maszenan A. M., Tay J.-H., Schumann P., Jiang H.-L., Tay S. T.-L. 2005; Quadrisphaera granulorum gen. nov., sp. nov. a Gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules. Int J Syst Evol Microbiol 55:1771–1777 [CrossRef]
    [Google Scholar]
  17. McCarthy A. J., Williams S. T. 1992; Actinomycetes as agents of biodegradation in the environment – a review. Gene 115:189–192 [CrossRef]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  20. Monciardini P., Sosio M., Cavaletti L., Chiocchini C., Donadio S. 2002; New PCR primers for the selective amplification of 16S rDNA from different groups of actinomycetes. FEMS Microbiol Ecol 42:419–429
    [Google Scholar]
  21. Monciardini P., Cavaletti L., Schumann P., Rohde M., Donadio S. 2003; Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria . Int J Syst Evol Microbiol 53:569–576 [CrossRef]
    [Google Scholar]
  22. Rainey F. A., Schumann P., Prauser H., Toalster R., Stackebrandt E. 1993; Sporichthya polymorpha represents a novel line of descent within the order Actinomycetales . FEMS Microbiol Lett 109:263–267 [CrossRef]
    [Google Scholar]
  23. Sait M., Hugenholtz P., Janssen P. H. 2002; Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. Environ Microbiol 4:654–666 [CrossRef]
    [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  26. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  27. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  28. Suter M. A. 1978; Isolierung von Melanin-negativen Mutanten aus Streptomyces glaucescens . PhD thesis Eidgenössische Technische Hochschule Zürich Switzerland (no. 6276: (in German
    [Google Scholar]
  29. Tamura T., Hayakawa M., Hatano K. 1998; A new genus of the order Actinomycetales , Cryptosporangium gen. nov., with descriptions of Cryptosporangium arvum sp.nov. and Cryptosporangium japonicum sp. nov. Int J Syst Bacteriol 48:995–1005 [CrossRef]
    [Google Scholar]
  30. Willems A., Moore W. E. C., Weiss N., Collins M. D. 1997; Phenotypic and phylogenetic characterization of some Eubacterium -like isolates containing a novel type B wall murein from human feces: description of Holdemania filiformis gen. nov., sp. nov. Int J Syst Bacteriol 47:1201–1204 [CrossRef]
    [Google Scholar]
  31. Zhang Z., Wang Y., Ruan J. 1997; A proposal to revive the genus Kitasatospora (Omura, Takahashi, Iwai, and Tanaka 1982). Int J Syst Bacteriol 47:1048–1054 [CrossRef]
    [Google Scholar]
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