1887

Abstract

A novel, Gram-positive bacterial strain was isolated from forest soil. Among species with validly published names, the 16S rRNA gene sequence is related most closely (approx. 93 % similarity) to that of DSM 43042. However, differently from this species, it forms both vegetative and aerial mycelia. The aerial hyphae are straight to slightly flexuous, starting to septate to form chains of more than 20 cylindrical spores with a rugose surface. The strain is acidophilic, with a pH range for robust growth between 4.3 and 6.8 and an optimum around 6.0. The peptidoglycan type is A3 -Dpm–Gly. The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. Predominant menaquinones are MK-9(H) and -9(H), and iso- and anteiso-branched C and C are the main cellular fatty acids. The DNA G+C content is 71.9 mol%. The distinct phylogenetic position and the unusual combination of chemotaxonomic characteristics justify the proposal of gen. nov., with the type species sp. nov. (type strain, ID139908 =DSM 44928=NRRL B-24433). fam. nov. is also proposed.

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2006-08-01
2019-10-14
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Field emission scanning electron microscopy (FESEM) of strain ID139908 grown on HSA5 agar for 3 weeks at 28 ºC. (a) Panoramic view of aerial hyphae. Arrows indicate bundles of hyphae running together. (b) Septation of hyphae gives origin to chains of more than 20 spores.

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Polar lipid composition of strain ID139908 , as determined by two-dimensional TLC. The polar lipids were identified as follows: 1, diphosphatidylglycerol; 2, phosphatidylglycerol; 3, phosphatidylinositol; 4, phosphatidylinositol mannosides; 5, 6, unidentified phospholipids.

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Neighbour-joining tree based on 1381 aligned positions within the 16S rRNA gene [ PDF] (105 KB)

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