1887

Abstract

The taxonomic position of two bovine strains, LMG 13603 and LMG 14595, assigned to the species on the basis of biochemical features, was reinvestigated. Both reference strains and two other isolates, 6/1 (=LMG 22829) originating from a charcoal-broiled river lamprey and IE38.4 (=LMG 22830) from the air of a poultry slaughter by-product processing plant, occupied a clearly separate position, on the basis of sequence analysis of the housekeeping gene (encoding the phenylalanyl-tRNA synthase -subunit), relative to the type strain of and all other enterococcal species with validly published names. 16S rRNA gene sequencing of strains LMG 13603, LMG 14595, 6/1 and IE38.4 confirmed their phylogenetic position in the species group, there being more than 99 % 16S rRNA gene sequence similarity to most members of the group, including , and revealed as the closest phylogenetic relative (99·8–99·9 %). Further phenotypic and genotypic analyses using whole-cell-protein electrophoresis, (GTG)-PCR fingerprinting, ribotyping and DNA–DNA hybridization experiments demonstrated that all four strains represent a novel enterococcal species, for which the name sp. nov. is proposed. The type strain is LMG 14595 (=CCM 7299).

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2005-11-01
2019-10-16
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vol. , part 6, pp. 2479 - 2484

Distance matrix tree based on 16S RNA gene sequence comparisons showing the phylogenetic relationships of sp. nov. with species included in the group and with selected enterococcal species representing the different phylogenetic lineages.

(GTG) -PCR fingerprints obtained from sp. nov. and phylogenetically related species of the group.

Ribotype patterns obtained with dIII (a) and RI (b) restriction enzymes from sp. nov. and representative strains of phylogenetically related species.

[PDF of Supplementary Figs S1-S3](554 KB)



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