1887

Abstract

Two bacterial strains isolated from different hospitals in Japan were subjected to a polyphasic analysis. Strains IFM 0803 and IFM 10383 were found to have morphological, biochemical, physiological and chemotaxonomic properties consistent with their classification in the genus . Strains IFM 0803 and IFM 10383 clustered with the type strain of , showing 16S rRNA gene sequence similarities of 98.6–98.9 % with this species. The novel strains could be distinguished from by a range of phenotypic properties. Based on their phenotypic and phylogenetic characteristics, the two isolates are proposed as members of a novel species of the genus sp. nov., with the type strain IFM 0803 (=NBRC 100660=JCM 12667=DSM 44883).

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2006-06-01
2024-04-25
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References

  1. Chapin K. C., Murray P. R. 1999; Stains. In Manual of Clinical Microbiology pp  1674–1686 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  3. Conville P. S., Brown J. M., Steigerwalt A. G., Lee J. W., Anderson V. L., Fishbain J. T., Holland S. M., Witebsky F. G. 2004; Nocardia kruczakiae sp. nov., a pathogen in immunocompromised patients and a member of the “ N. nova complex”. J Clin Microbiol 42:5139–5145 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Goodfellow M. 1998; Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections, 9th edn., vol. 2, Systematic Bacteriology pp  463–489 Edited by Balows A., Duerden B. I. London: Arnold;
    [Google Scholar]
  6. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312:47–82
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  8. Hitti W., Wolff M. 2005; Two cases of multidrug-resistant Nocardia farcinica infection in immunosuppressed patients and implications for empiric therapy. Eur J Clin Microbiol Infect Dis 24:142–144 [CrossRef]
    [Google Scholar]
  9. Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. 2004; The complete genomic sequence of Nocardia farcinica IFM 10152. Proc Natl Acad Sci U S A 101:14925–14930 [CrossRef]
    [Google Scholar]
  10. Kageyama A., Torikoe K., Iwamoto M. & 7 other authors (2004a). Nocardia arthritidis sp. nov., a new pathogen isolated from a patient with rheumatoid arthritis in Japan. J Clin Microbiol 42:2366–2371 [CrossRef]
    [Google Scholar]
  11. Kageyama A., Poonwan N., Yazawa K., Mikami Y., Nishimura K. 2004b; Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. Int J Syst Evol Microbiol 54:125–130 [CrossRef]
    [Google Scholar]
  12. Kageyama A., Yazawa K., Ishikawa J., Hotta K., Nishimura K., Mikami Y. 2004c; Nocardial infections in Japan from 1992 to 2001, including the first report of infection by Nocardia transvalensis . Eur J Epidemiol 19:383–389
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  14. Kimura M., Ota T. 1972; On the stochastic model for estimation of mutational distance between homologous proteins. J Mol Evol 2:87–90 [CrossRef]
    [Google Scholar]
  15. Kubica G. P., Beam R. E. 1961; The arylsulfatase activity of acid-fast bacilli. II. The differentiation of Mycobacterium avium from the unclassified group III nonphoto chromogenic mycobacteria. Am Rev Resp Dis 83:733–736
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp  227–291 Edited by Dietz A., Thayer D. W. Fairfax, VA: Society for Industrial Microbiology;
    [Google Scholar]
  17. Mikami Y., Yazawa K. 1989; Susceptibility patterns of pathogenic Nocardia to some selected antimicrobial agents and their usefulness in the identification work in a clinical laboratory. Bull JFCC 5:89–95
    [Google Scholar]
  18. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  19. Miyadoh M. 2001; Identification procedure at the genus level. In Identification Manual of Actinomycetes pp  9–19 Edited by Miyadoh S., Hamada M., Hotta K., Kudo T., Seino A., Suzuki K., Yokota A. Tokyo: Business Center for Academic Societies Japan;
    [Google Scholar]
  20. Poonwan N., Kusum M., Mikami Y., Yazawa K., Tanaka K., Gonoi T., Hasegawa S., Konyama K. 1995; Pathogenic Nocardia isolated from clinical specimens including those of AIDS patients in Thailand. Eur J Epidemiol 11:507–512 [CrossRef]
    [Google Scholar]
  21. Poonwan N., Mekha N., Yazawa K., Thunyaharn S., Yamanaka A., Mikami Y. 2005; Characterization of clinical isolates of pathogenic Nocardia strains and related actinomycetes in Thailand from 1996 to 2003. Mycopathologia 159:361–368 [CrossRef]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Zhang J., Liu Z., Goodfellow M. 2004; Nocardia xishanensis sp. nov., a novel actinomycete isolated from soil. Int J Syst Evol Microbiol 54:2301–2305 [CrossRef]
    [Google Scholar]
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