1887

Abstract

Four nitrite-dissimilating strains, isolated from Weser Estuary sediments, were investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the ‘’ and are related to the genus . The highest level of sequence similarity (100 %) was found with strain M3A (=ATCC 700596), a dimethyl sulfide-producing marine isolate that was included in this study. DNA–DNA hybridizations between the five strains and related strains confirmed that the former belong to a single and novel species within the genus . The isolates are Gram-negative, motile, rod-shaped cells with a DNA G+C content of about 56 mol%. The whole-cell fatty acid profiles of the isolates were very similar and included C, C cyclo, C 7, summed feature 2 (comprising any combination of C aldehyde, an unknown fatty acid of equivalent chain length 10·928, C iso I and C 3-OH) and summed feature 3 (C iso 2-OH and/or C 7) as the major fatty acid components. On the basis of their phylogenetic, genomic and phenotypic properties, the five novel strains can be assigned to the genus as a novel species, for which the name sp. nov. is proposed. The type strain is LMG 22996 (=CCUG 50924).

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2005-11-01
2021-02-27
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