RT Journal Article SR Electronic(1) A1 Mayilraj, S. A1 Krishnamurthi, S. A1 Saha, P. A1 Saini, H. S.YR 2006 T1 Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 3 SP 519 OP 522 DO https://doi.org/10.1099/ijs.0.63836-0 PB Microbiology Society, SN 1466-5034, AB Polyphasic characterization of an actinomycete strain VT-36T isolated from a sugar-cane field soil sample collected in Punjab State, India, revealed that the strain belongs to the genus Kitasatospora. The strain's chemotaxonomic characters and G+C content of DNA (76·5 mol%) were typical of members of the genus. Analysis of the 16S rRNA gene sequence supported the generic affiliation of the strain and showed that its closest phylogenetic relative was Kitasatospora putterlickiae F18-98T (=DSM 44665T) (98·3 % 16S rRNA gene sequence similarity). The similarities with type strains of all other Kitasatospora species were in the range 95·1–97·0 %. The results of DNA–DNA hybridization showed 54 % relatedness of the isolate and K. putterlickiae F18-98T. Based on the above data and the phenotypic differences from K. putterlickiae and other Kitasatospora species, it is proposed that the isolate should be classified as the type strain of a novel species, Kitasatospora sampliensis sp. nov., with strain VT-36T (=MTCC 6546T=DSM 44898T=JCM 13010T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63836-0