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Abstract

Two Gram-negative, motile, non-spore-forming, slightly halophilic bacteria (strains DSW10-10 and DSW10-19) were isolated from sea water and subjected to a polyphasic taxonomic study. They grew optimally at 30 °C and in the presence of 2–3 % (w/v) NaCl. Strains DSW10-10 and DSW10-19 were characterized chemotaxonomically as containing Q-8 as the predominant ubiquinone and C 7, C and C 7 and/or iso-C 2-OH as the major fatty acids. Their DNA G+C contents were 45·3–45·7 mol%. Strains DSW10-10 and DSW10-19 exhibited a 16S rRNA gene sequence similarity value of 100 % and possessed a mean DNA–DNA relatedness level of 85 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains DSW10-10 and DSW10-19 fell within the radiation of the cluster encompassed by the genus . Strains DSW10-10 and DSW10-19 had 16S rRNA gene sequence similarity levels of 95·7–97·7 % with respect to the type strains of species with validly published names. Levels of DNA–DNA relatedness were low enough to indicate that the two strains constitute a distinct species. On the basis of phenotypic data and phylogenetic and genetic distinctiveness, strains DSW10-10 (=KCTC 12394=DSM 17202) and DSW10-19 were placed in the genus as members of a novel species, sp. nov.

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2005-11-01
2020-01-24
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