1887

Abstract

Among basidiomycetous yeast strains isolated from wilting leaves of various plants in China, two groups of strains were distinguished from the others by morphological and physiological characterization. Molecular taxonomic analysis based on sequencing of the large subunit (26S) rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region confirmed that the two groups represent two novel species. They are proposed as sp. nov. (type strain WS 6.4=AS 2.2493=CBS 10077) and sp. nov. (type strain SH 64=AS 2.2523=CBS 10075). The phylogenetic relationships of the novel species with described species and related ustilagomycetous teleomorphs were analysed based on the combined sequences of ITS and D1/D2. The phenotypic diagnosis of was emended because of the negative inositol assimilation reaction of sp. nov., which was closely related to the type species of the genus, .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63827-0
2006-01-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/289.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63827-0&mimeType=html&fmt=ahah

References

  1. Bai, F.-Y., Zhao, J.-H., Takashima, M., Jia, J.-H., Boekhout, T. & Nakase, T. ( 2002; ). Reclassification of the Sporobolomyces roseus and the Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 52, 2309–2314.[CrossRef]
    [Google Scholar]
  2. Begerow, D. & Bauer, R. ( 2000; ). Phylogenetic placements of ustilaginomycetous anamorphs as deduced from nuclear LSU rDNA sequences. Mycol Res 104, 53–60.[CrossRef]
    [Google Scholar]
  3. Boekhout, T. ( 1987; ). Systematics of anamorphs of Ustilaginales (smut fungi) – a preliminary survey. Stud Mycol 30, 137–149.
    [Google Scholar]
  4. Boekhout, T. ( 1995; ). Pseudozyma Bandoni emend. Boekhout, a genus for yeast-like anamorphs of ustilaginales. J Gen Appl Microbiol 41, 359–366.[CrossRef]
    [Google Scholar]
  5. Boekhout, T. & Fell, J. W. ( 1998; ). Pseudozyma Bandoni emend. Boekhout, and a comparison with the yeast state of Ustilago maydis (De Candolle) Corda. In The Yeasts, a Taxonomic Study, 4th edn, pp. 790–797. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  6. Boekhout, T., Fell, J. W. & O'Donnell, K. ( 1995; ). Molecular systematics of some yeast-like anamorphs belonging to the Ustilaginales and Tilletiales. Stud Mycol 38, 175–183.
    [Google Scholar]
  7. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Makimura, K., Murayama, Y. S. & Yamaguchi, H. ( 1994; ). Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40, 358–364.[CrossRef]
    [Google Scholar]
  11. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis, and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  12. Nakase, T. & Takashima, M. ( 1993; ). A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Rev 3, 33–34.
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Stoll, M., Begerow, D. & Oberwinkler, F. ( 2005; ). Molecular phylogeny of Ustilago, Sporisorium, and related taxa based on combined analysis of rDNA sequences. Mycol Res 109, 342–356.[CrossRef]
    [Google Scholar]
  15. Sugita, T., Takashima, M., Poonwan, N., Mekha, N., Malaithao, K., Thungmauthasawat, B., Prasarn, S., Luangsook, P. & Kudo, T. ( 2003; ). The first isolation ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients' blood and a description of two new species: P. parantarctica and P. thailandica. Microbiol Immunol 47, 183–190.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Yamada, Y. & Kondo, K. ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19, 59–77.[CrossRef]
    [Google Scholar]
  18. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63827-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63827-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error