1887

Abstract

Actinomycete strain VC-A46, which exhibits antifungal activity, was isolated from a soil sample collected from Cheonan, Korea. The strain was determined to belong to the genus , based on its morphological and chemotaxonomic characteristics. The cell wall of this strain contained -diaminopimelic acid. The predominant fatty acids were iso-C, C and C 9. The DNA G+C content of strain VC-A46 was 75·5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of this strain revealed that it differed from those of the described streptomycetes available in public databases. Analyses of DNA–DNA relatedness data, together with data regarding the strain's morphological, physiological and biochemical characteristics, also confirmed that this strain constitutes a novel taxon, which is distinguishable from closely related reference strains. Therefore, it is proposed that strain VC-A46 (=KCCM 42119=NBRC 100940) represents the type strain of a novel species, named sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63816-0
2006-02-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/2/471.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63816-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Becker, B., Lechevalier, M. P. & Lechevalier, H. A. ( 1965; ). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243.
    [Google Scholar]
  3. Chun, J. S., Youn, H. D., Yim, Y. I., Lee, H. K., Kim, M. Y., Hah, Y. C. & Kang, S. O. ( 1997; ). Streptomyces seoulensis sp. nov. Int J Syst Bacteriol 47, 492–498.[CrossRef]
    [Google Scholar]
  4. Chung, Y. R., Sung, K. C., Mo, H. K., Son, D. Y., Nam, J. S., Chun, J. S. & Bae, K. S. ( 1999; ). Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Int J Syst Bacteriol 49, 753–758.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Guckert, J. B., Ringelberg, D. B., White, D. C., Hanson, R. S. & Bratina, B. J. ( 1991; ). Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the Proteobacteria. J Gen Microbiol 137, 2631–2641.[CrossRef]
    [Google Scholar]
  7. Johnson, J. L. ( 1985; ). Determination of DNA base composition. Methods Microbiol 18, 1–31.
    [Google Scholar]
  8. Kim, B. S. & Hwang, B. K. ( 2003; ). Biofungicides. In Fungal Biotechnology in Agricultural, Food and Environmental Applications, pp. 123–133. Edited by D. K. Arora. New York: Marcel Dekker.
  9. Labeda, D. P. ( 1993; ). DNA relatedness among strains of the Streptomyces lavendulae phenotypic cluster group. Int J Syst Bacteriol 43, 822–825.[CrossRef]
    [Google Scholar]
  10. Labeda, D. P. ( 1996; ). DNA relatedness among verticil-forming Streptomyces species (formerly Streptoverticillium species). Int J Syst Bacteriol 46, 699–703.[CrossRef]
    [Google Scholar]
  11. Labeda, D. P. ( 1998; ). DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48, 829–832.[CrossRef]
    [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  13. Lee, J. Y. & Hwang, B. K. ( 2002; ). Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can J Microbiol 48, 407–417.[CrossRef]
    [Google Scholar]
  14. Lee, J. Y., Lee, J. Y., Jung, H. W. & Hwang, B. K. ( 2005; ). Streptomyces koyangensis sp. nov., a novel actinomycete that produces 4-phenyl-3-butenoic acid. Int J Syst Evol Microbiol 55, 257–262.[CrossRef]
    [Google Scholar]
  15. Locci, R. ( 1989; ). Streptomycetes and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2452. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Mandel, M., Igambi, L., Bergendahl, M. L., Dodson, M. L., Jr & Scheltgen, E. ( 1970; ). Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101, 333–338.
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  19. Pospiech, A. & Neumann, B. ( 1995; ). A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet 11, 217–218.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Swofford, D. L. ( 2002; ). paup*. Phylogenetic Analysis Using Parsimony (* and Other Methods), version 4.0b10. Sunderland, MA: Sinauer Associates.
  23. Tanaka, Y. T. & Ōmura, S. ( 1993; ). Agroactive compounds of microbial origin. Annu Rev Microbiol 47, 57–87.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  25. Waksman, A. S. & Henrici, A. T. ( 1943; ). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341.
    [Google Scholar]
  26. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  27. Williams, S. T. & Davies, F. L. ( 1967; ). Use of scanning electron microscope for the examination of actinomycetes. J Gen Microbiol 48, 171–177.[CrossRef]
    [Google Scholar]
  28. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  29. Wu, W., Welsh, M. J., Kaufman, P. B. & Zhang, H. H. ( 1997; ). Subcloning of DNA fragments. In Methods in Gene Biotechnology, pp. 15–28. Boca Raton, FL: CRC Press.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63816-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63816-0
Loading

Data & Media loading...

Supplements

[PDF file of Supplementary Tables S1 and S2](15 KB)

PDF

Scanning electron micrograph of spore chains of strain VC-A46 cultured on yeast extract/malt extract agar for 7 days. Bar, 1 µm.

IMAGE

Phylogenetic tree showing the relationship between strain VC-A46 and representative species of the genus based on the variable γ region sequences of the 16S rRNA gene. [PDF](40 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error