Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica
A novel obligately anaerobic, proteolytic bacterium, designated AP15T, was isolated from lake sediments of Schirmacher Oasis, Antarctica. The bacterium produced maximum cell mass between 5 and 10 °C in an anaerobic basal medium containing 0·5 % tryptone and peptone. The strain grew optimally at a pH around 8·0 and tolerated NaCl up to a concentration of 7·5 %. It contained diphosphatidylglycerol as the major phospholipid and C15 : 0, C16 : 0 and C17 : 0 as the major cellular fatty acids. Several amino acids, including arginine, leucine, isoleucine, cysteine, glutamate and serine, supported growth. Glutamate was degraded to acetate, propionate, CO2 and H2. In addition, the strain degraded carbohydrates including glucose, raffinose, adonitol, ribose and rhamnose. The main fermentation products during growth on glucose were H2, CO2, formate, acetate, propionate and isovalerate. The DNA G+C content of the bacterium was 24 mol%. On the basis of a phylogenetic analysis, strain AP15T is identified as a close relative of Clostridium subterminale ATCC 25774T, with which it shares 99·5 % similarity at the 16S rRNA gene sequence level; however, it exhibits a low DNA–DNA binding value (55 %) to this strain at the whole-genome level. In addition to showing other major differences with respect to C. subterminale and other members of the genus Clostridium, AP15T also exhibits phenotypic differences. On the basis of these differences, strain AP15T is identified as representing a novel species of the genus Clostridium, for which the name Clostridium schirmacherense sp. nov. is proposed. The type strain is AP15T (=DSM 17394T=JCM 13289T).
AttwoodG. T.,
ReillyK.,
PatelB. K. C.1996; Clostridium proteoclasticum sp. nov., a novel proteolytic bacterium from the bovine rumen. Int J Syst Bacteriol 46:753–758[CrossRef]
CatoE. P.,
GeorgeW. L.,
FinegoldS. M.1986; Genus Clostridium Prazmowski 1880, 23AL
. In Bergey's Manual of Systematic Bacteriologyvol. 2 pp 1141–1200 Edited by
SneathP. H. A.,
MairN. S.,
SharpeM. E.,
HoltJ. G.
Baltimore: William & Wilkins;
CollinsM. D.,
LawsonP. A.,
WillemsA.,
CordobaJ. J.,
Fernandez- GarayzabalJ.,
GarciaP.,
CaiJ.,
HippeH.,
FarrowJ. A. E.1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826[CrossRef]
FelsensteinJ.1993phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
FranzmannP. D.,
RobertsN. J.,
MancusoC. A.,
BurtonH. R.,
McMeekinT. A.1991; Methane production in meromictic Ace Lake, Antarctica. Hydrobiologia 210:191–201[CrossRef]
HallJ. D.,
McCroskeyL. M.,
PincombB. J.,
HathewayC. L.1985; Isolation of an organism resembling Clostridium baratii which produces type F botulinal toxin from an infant with botulism. J Clin Microbiol 21:654–655
KimuraM.1980; Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
MechichiT.,
LabatM.,
PatelB. K. C.,
WooT. H. S.,
ThomasP.,
GarciaJ. L.1999; Clostridium methoxybenzovorans sp. nov., a new aromatic o -demethylating homoacetogen from an olive mill wastewater treatment digester. Int J Syst Bacteriol 49:1201–1209[CrossRef]
MountfortD. O.,
RhodesL. L.1991; Anaerobic growth and fermentation characteristics of Paecilomyces lilacinus isolated from mullet gut. Appl Environ Microbiol 57:1963–1968
ReddyG. S. N.,
AggarwalR. K.,
MatsumotoG. I.,
ShivajiS.2000; Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50:1553–1561[CrossRef]
ShivajiS.,
RaoN. S.,
SaisreeL.,
ReddyG. S. N.,
KumarG. S.,
BhargavaP. M.1989; Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10:225–229
ShivajiS.,
RayM. K.,
RaoN. S.,
SaisreeL.,
JagannadhamM. V.,
KumarG. S.,
ReddyG. S. N.,
BhargavaP. M.1992; Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–106[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by
GerhardtP.
Washington, DC: American Society for Microbiology;
SuenJ. C.,
HathewayC. L.,
SteigerwaltA. G.,
BrennerD. J.1988; Clostridium argentinense sp. nov.: a genetically homogeneous group composed of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme
. Int J Syst Bacteriol 38:375–381[CrossRef]
Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica