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Abstract

Two proteolytic, strictly anaerobic bacterial strains (TB107 and TB6-6) were isolated from the granule sludge of an upflow anaerobic sludge blanket reactor treating brewery wastewater. The strains were Gram-negative, non-spore-forming and motile. Cells were rod-shaped (0·6–0·9×1·9–2·2 μm). Growth of the strains was observed at 20–45 °C and pH 6·0–9·7. The strains were proteolytic. Yeast extract, peptone, pyruvate, glycine and -arginine could be used as carbon and energy sources. Weak growth was also observed with tryptone, -serine, -threonine and -alanine as carbon and energy sources. Both strains did not use any of the tested carbohydrates, alcohols and fatty acids except pyruvate. Acetic acid and NH were produced from yeast extract, peptone and -arginine, and propionic acid was also produced from yeast extract. Pyruvate was converted to acetic acid and CO. Gelatin was not hydrolysed. Indole and HS were not produced. The two strains did not grow in medium containing 20 % bile. Addition of strain TB107 to a syntrophic propionate-degrading co-culture accelerated the propionate-degradation rate. The predominant cellular fatty acid was the branched-chain fatty acid anteiso-C (46·21 %). The genomic DNA G+C contents of strains TB107 and TB6-6 were 46·6 and 48·9 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represent a new phyletic sublineage within the (CFB) group, with <91 % 16S rRNA gene sequence similarity to the closest species with validly published names. On the basis of polyphasic evidence from this study, a new genus and species, gen. nov., sp. nov., is proposed, with strain TB107 (=JCM 12891=AS 1.5024) as the type strain.

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2005-11-01
2019-10-22
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References

  1. Chen, S. & Dong, X. ( 2004; ). Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 54, 2257–2262.[CrossRef]
    [Google Scholar]
  2. Chen, S., Liu, X. & Dong, X. ( 2005; ). Syntrophobacter sulfatireducens sp. nov., a novel syntrophic, propionate-oxidizing bacterium isolated from UASB reactors. Int J Syst Evol Microbiol 55, 1319–1324.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Hofstad, T., Olsen, I., Eribe, E. R., Falsen, E., Collins, M. D. & Lawson, P. A. ( 2000; ). Dysgonomonas gen. nov. to accommodate Dysgonomonas gadei sp. nov., an organism isolated from a human gall bladder, and Dysgonomonas capnocytophagoides (formerly CDC group DF-3). Int J Syst Evol Microbiol 50, 2189–2195.[CrossRef]
    [Google Scholar]
  6. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  7. Holdeman, L. V., Kelley, R. W. & Moore, W. E. C. ( 1984; ). Genus I. Bacteroides Castellani and Chalmers 1919, 959AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 604–631. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  8. Hungate, R. E. ( 1969; ). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  9. Johnson, J. L., Moore, W. E. C. & Moore, L. V. H. ( 1986; ). Bacteroides caccae sp. nov., Bacteroides merdae sp. nov., and Bacteroides stercoris sp. nov. isolated from human feces. Int J Syst Bacteriol 36, 499–501.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  12. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  13. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  14. Moore, L. V. H., Bourne, D. M. & Moore, W. E. C. ( 1994; ). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic Gram-negative bacilli. Int J Syst Bacteriol 44, 338–347.[CrossRef]
    [Google Scholar]
  15. Paster, B. J., Dewhirst, F. E., Olsen, I. & Fraser, G. J. ( 1994; ). Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria. J Bacteriol 176, 725–732.
    [Google Scholar]
  16. Reynolds, E. S. ( 1963; ). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17, 208–212.[CrossRef]
    [Google Scholar]
  17. Sakamoto, M., Suzuki, M., Umeda, M., Ishikawa, I. & Benno, Y. ( 2002; ). Reclassification of Bacteroides forsythus ( Tanner et al. 1986 ) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52, 841–849.[CrossRef]
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  19. Schink, B. ( 1997; ). Energetics of syntrophic cooperation in methanogenic degradation. Microbiol Mol Biol Rev 61, 262–280.
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Woods & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Tanner, A. C. R., Listgarten, M. A., Ebersole, J. L. & Strzempko, M. N. ( 1986; ). Bacteroides forsythus sp. nov., a slow-growing, fusiform Bacteroides sp. from the human oral cavity. Int J Syst Bacteriol 36, 213–221.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
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