1887

Abstract

An eicosapentaenoic acid-producing bacterium, previously described as sp. strain SCRC-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16S rRNA gene sequence analysis and DNA–DNA hybridization. The isolate was Gram-negative, rod-shaped and motile by using polar flagella. The strain grew at 4–32 °C; the optimum growth temperature was 27 °C. NaCl was required for growth. The major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its DNA G+C content was 42·8 mol%. The whole-cell fatty acids mainly (above 5 %) consisted of iso-C, iso-C, C, C 7, C 7 and C 3 (eicosapentaenoic acid). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCRC-2738 was related closely (sequence similarities above 99 %) to (99·3 %), (99·3 %) and (99·2 %). DNA–DNA hybridization and phenotypic characteristics confirmed that strain SCRC-2738 merited classification as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCRC-2738 (=JCM 13187=NCIMB 14060).

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2005-11-01
2019-10-20
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