Two Gram-negative, motile, non-spore-forming and rod-shaped bacterial strains, DS-123 and DS-140, were isolated from soil and their taxonomic positions were investigated by a polyphasic study. Strains DS-123 and DS-140 grew optimally at 30 °C and pH 6·5 without NaCl. They contained Q-8 as the predominant ubiquione and iso-C 9, iso-C and iso-C as the major fatty acids. Major polar lipids detected in the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an amino-group-containing lipid that was ninhydrin-positive. Their DNA G+C contents were 71·0 mol%. Strains DS-123 and DS-140 exhibited no difference in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 92 %. Phylogenetic trees based on 16S rRNA gene sequences showed that strains DS-123 and DS-140 formed a distinct evolutionary lineage within the . The 16S rRNA gene sequences of strains DS-123 and DS-140 exhibited similarity values of less than 94·1 % to members of the . Strains DS-123 and DS-140 were distinguished from phylogenetically related genera by differences in some phenotypic properties. On the basis of phenotypic, phylogenetic and genetic data, it is proposed that strains DS-123 (=KCTC 12396=DSM 17203) and DS-140 be classified in a novel genus and species, gen. nov., sp. nov.


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vol. , part 1, pp. 145-150

Transmission electron micrograph of strain DS-123 . [PDF](178 KB)

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