1887

Abstract

A Gram-positive, rod-shaped, motile and endospore-forming bacterial strain, KSL-134, was isolated from an alkaline soil in Korea, and its taxonomic position was investigated by a polyphasic study. Strain KSL-134 grew optimally at pH 7·5 and 30 °C. Its cell wall peptidoglycan contained -diaminopimelic acid. Strain KSL-134 was characterized as having MK-7 as the predominant menaquinone and anteiso-C as the major fatty acid. The DNA G+C content was 49·4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KSL-134 formed a distinct lineage within the evolutionary radiation encompassed by the genus . Similarity levels between the 16S rRNA gene sequence of strain KSL-134 and those of the type strains of recognized species ranged from 90·4 to 96·5 %. DNA–DNA relatedness levels and some differential phenotypic properties were enough to distinguish strain KSL-134 from several phylogenetically related species. On the basis of phenotypic and phylogenetic data, strain KSL-134 (=KCTC 3956=DSM 17040) was classified in the genus as a member of a novel species, for which the name sp. nov. is proposed.

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2005-11-01
2019-12-05
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vol. , part 6, pp. 2339-2344

Neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic positions of sp. nov., species and representatives of some other related taxa. [PDF](29 KB)



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