1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 55, Issue 6

sp. nov., isolated from an alkaline soil in Korea Free

Abstract

A Gram-positive, rod-shaped, motile and endospore-forming bacterial strain, KSL-134, was isolated from an alkaline soil in Korea, and its taxonomic position was investigated by a polyphasic study. Strain KSL-134 grew optimally at pH 7·5 and 30 °C. Its cell wall peptidoglycan contained -diaminopimelic acid. Strain KSL-134 was characterized as having MK-7 as the predominant menaquinone and anteiso-C as the major fatty acid. The DNA G+C content was 49·4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KSL-134 formed a distinct lineage within the evolutionary radiation encompassed by the genus . Similarity levels between the 16S rRNA gene sequence of strain KSL-134 and those of the type strains of recognized species ranged from 90·4 to 96·5 %. DNA–DNA relatedness levels and some differential phenotypic properties were enough to distinguish strain KSL-134 from several phylogenetically related species. On the basis of phenotypic and phylogenetic data, strain KSL-134 (=KCTC 3956=DSM 17040) was classified in the genus as a member of a novel species, for which the name sp. nov. is proposed.

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63771-0
2005-11-01
2023-03-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/6/2339.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63771-0&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260
     [Google Scholar]
  2. Baumann L., Baumann P. 1981; The marine Gram-negative eubacteria; genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes . A Handbook on Habitats, Isolation, and Identification of Bacteria pp  1302–1330 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  3. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1105–1140 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
     [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  6. Dasman Kajiyama S., Kawasaki H., Yagi M., Seki T., Fukusaki E., Kobayashi A. 2002; Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune . Int J Syst Evol Microbiol 52:1669–1674 [CrossRef]
     [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  8. Heyndrickx M., Vandemeulebroecke K., Scheldeman B., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1996; A polyphasic reassessment of the genus Paenibacillus , reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb.nov. and of Bacillus peoriae (Montefusco et al . 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae . Int J Syst Bacteriol 46:988–1003 [CrossRef]
     [Google Scholar]
  9. Kanzawa Y., Harada A., Takeuchi M., Yokota A., Harada T. 1995; Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyze resistant curdlan. Int J Syst Bacteriol 45:515–521 [CrossRef]
     [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
     [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  12. Nakamura L. K. 1990; Bacillus thiaminolyticus sp. nov., nom. rev. Int J Syst Bacteriol 40:242–246 [CrossRef]
     [Google Scholar]
  13. Nakamura L. K. 1996; Paenibacillus apiarius sp. nov. Int J Syst Bacteriol 46:688–693 [CrossRef]
     [Google Scholar]
  14. Pettersson B., Rippere K. E., Yousten A. A., Priest F. G. 1999; Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov. Int J Syst Bacteriol 49:531–540 [CrossRef]
     [Google Scholar]
  15. Rivas R., Mateos P. F., Martínez-Molina E., Velázquez E. 2005a; Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55:405–408 [CrossRef]
     [Google Scholar]
  16. Rivas R., Mateos P. F., Martínez-Molina E., Velázquez E. 2005b; Paenibacillus phyllosphaerae sp. nov., a xylanolytic bacterium isolated from phyllosphere of Phoenix dactylifera . Int J Syst Evol Microbiol 55:743–746 [CrossRef]
     [Google Scholar]
  17. Rivas R., Gutiérrez C., Abril A., Mateos P. F., Martínez-Molina E., Ventosa A., Velázquez E. 2005c; Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum . Int J Syst Evol Microbiol 55:1305–1309 [CrossRef]
     [Google Scholar]
  18. Roux V., Raoult D. 2004; Paenibacillus massiliensis sp. nov., Paenibacillus sanguinis sp. nov. and Paenibacillus timonensis sp. nov., isolated from blood cultures. Int J Syst Evol Microbiol 54:1049–1054 [CrossRef]
     [Google Scholar]
  19. Sánchez M. M., Fritze D., Blanco A., Spröer C., Tindall B. J., Schumann P., Kroppenstedt R. M., Diaz P., Pastor F. I. J. 2005; Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55:935–939 [CrossRef]
     [Google Scholar]
  20. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI;
     [Google Scholar]
  21. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997a; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
     [Google Scholar]
  22. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997b; Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol 47:299–306 [CrossRef]
     [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  24. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
     [Google Scholar]
  25. Takeda M., Suzuki I., Koizumi J. 2005; Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans . Int J Syst Evol Microbiol 55:737–741 [CrossRef]
     [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  27. Uetanabaro A. P., Wahrenburg C., Hunger W., Pukall R., Spröer C., Stackebrandt E., de Canhos V. P., Claus D., Fritze D. 2003; Paenibacillus agarexedens sp. nov., nom. rev., and Paenibacillus agaridevorans sp. nov. Int J Syst Evol Microbiol 53:1051–1057 [CrossRef]
     [Google Scholar]
  28. van der Maarel M. J. E. C., Veen A., Wijbenga D. J. 2000; Paenibacillus granivorans sp. nov., a new Paenibacillus species which degrades native potato starch granules. Syst Appl Microbiol 23:344–348 [CrossRef]
     [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
     [Google Scholar]
  31. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
     [Google Scholar]
  32. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63771-0
Loading
Paenibacillus alkaliterrae sp. nov., isolated from an alkaline soil in Korea
Int J Syst Evol Microbiol 55, 2339 (2005); https://doi.org/10.1099/ijs.0.63771-0
/content/journal/ijsem/10.1099/ijs.0.63771-0
/content/journal/ijsem/10.1099/ijs.0.63771-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error