1887

Abstract

A novel extremely halophilic strain, isolated from Aibi salt lake, Xin-Jiang, China, was subjected to polyphasic taxonomic characterization. This strain, designated AB14, is neutrophilic, motile and requires at least 10 % (w/v) NaCl for growth. Strain AB14 grows at 24–58 °C, with optimal growth at 42–45 °C. Mg is not required, but growth is observed in MgCl concentrations as high as 1·0 M. Strain AB14 possesses the diphytanyl (CC) and phytanyl-sesterterpanyl diether (CC) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6 disulfate 1→2 glucose-glycerol diether. The genomic DNA G+C content is 66·6 mol%. The 16S rRNA gene sequence similarity values of strain AB14 with its nearest phylogenetic neighbours ( and ) are 98·6 and 96·0 %, respectively. DNA–DNA hybridization revealed 54 % relatedness between strain AB14 and JCM 11050 and 21 % between strain AB14 and JCM 9101. It is therefore proposed that strain AB14 represents a novel species, for which the name sp. nov. is proposed. The type strain is AB14 (=AS 1.3730=JCM 12889).

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2005-11-01
2019-10-21
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References

  1. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 143–153.[CrossRef]
    [Google Scholar]
  2. Dussault, H. P. ( 1955; ). An improved technique for staining red halophilic bacteria. J Bacteriol 70, 484–485.
    [Google Scholar]
  3. Feng, J., Zhou, P., Zhou, Y.-G., Liu, S.-J. & Warren-Rhodes, K. ( 2005; ). Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Int J Syst Evol Microbiol 55, 149–152.[CrossRef]
    [Google Scholar]
  4. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  5. Kamekura, M. & Kates, M. ( 1988; ). Lipids of halophilic archaebacteria. In Halophilic Bacteria II, pp. 25–54. Edited by F. Rodriguez-Valera. Boca Raton: CRC Press.
  6. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  7. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  8. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4, 109–118.
    [Google Scholar]
  9. McGenity, T. J., Gemmell, R. T. & Grant, W. D. ( 1998; ). Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom. nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48, 1187–1196.[CrossRef]
    [Google Scholar]
  10. Montalvo-Rodríguez, R., López-Garriga, J., Vreeland, R. H., Oren, A., Ventosa, A. & Kamekura, M. ( 2000; ). Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. Int J Syst Evol Microbiol 50, 1065–1071.[CrossRef]
    [Google Scholar]
  11. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  12. Oren, A., Elevi, R., Watanabe, S., Ihara, K. & Corcelli, A. ( 2002; ). Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei. Int J Syst Evol Microbiol 52, 1831–1835.[CrossRef]
    [Google Scholar]
  13. Sehgal, S. N. & Gibbons, N. E. ( 1960; ). Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6, 165–169.[CrossRef]
    [Google Scholar]
  14. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal_w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  15. Tindall, B. J. ( 2003; ). Taxonomic problems arising in the genera Haloterrigena and Natrinema. Int J Syst Evol Microbiol 53, 1697–1698.[CrossRef]
    [Google Scholar]
  16. Ventosa, A., Gutierrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
  17. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, T. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
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vol. , part 6, pp. 2539-2542

Thin-layer chromatogram of lipids from strain AB14 and JCM 11050 . [PDF](151 KB)



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