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Abstract

Two strains of free-living diazotrophs isolated from soil from a rice paddy field were characterized by using a polyphasic approach. The novel strains, A-7 and A-4, were found to be very closely related, with 99·9 % 16S rRNA gene sequence similarity and a DNA–DNA hybridization value of 89·5 %, suggesting that they represent a single species. 16S rRNA gene sequence analyses indicated that the two strains fell within the lineage, with less than 96·7 % sequence similarity to other species. Chemotaxonomic characteristics of the novel strains, including DNA G+C content (65·1 mol%), the major quinone system (Q-8), predominant fatty acids (16 : 17 and 16 : 0) and major hydroxy fatty acids (3-OH 10 : 0 and 3-OH 12 : 0), are similar to those of the genus . The novel strains showed positive results for floc formation which is accepted as confirmatory for species of the genus . However, the novel strains can be distinguished from the other species of by physiological characteristics. The name sp. nov. is therefore proposed for the novel strains with strain A-7 (=IAM 15218=CCTCC AB 2052005) as the type strain. Phylogenetic and chemotaxonomic analyses indicate that strain ATCC 19623, designated as a reference strain of , does not belong to the genus but to a new genus of . The name gen. nov., sp. nov. is proposed for strain ATCC 19623 (=IAM 12669).

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2006-03-01
2019-10-23
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