@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63751-0, author = "Denner, Ewald B. M. and Kolari, Marko and Hoornstra, Douwe and Tsitko, Irina and Kämpfer, Peter and Busse, Hans-Jürgen and Salkinoja-Salonen, Mirja", title = "Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderately thermophilic bacterium isolated from coloured slime deposits in paper machines", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2006", volume = "56", number = "6", pages = "1355-1362", doi = "https://doi.org/10.1099/ijs.0.63751-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63751-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pNP, p-nitrophenyl", keywords = "PUT, putrescine", keywords = "HSPD, sym-homospermidine", keywords = "pNA, p-nitroanilide", keywords = "SPD, spermidine", abstract = "Six red-pigmented strains of the Alphaproteobacteria with optimal growth between 45 and 54 °C were previously isolated from coloured biofilms in two fine-paper machines and one pulp dryer. The strains were found to be resistant to 15 p.p.m. 2,2-dibromo-3-nitrilopropionamide, a common industrial biocide. 16S RNA gene sequence similarity of the isolates was 99.7–100 %. Ribotyping using the restriction enzymes PvuII and EcoRI showed that four of the isolates (C-lvk-R2A-1, C-lvk-R2A-2T, C-R2A-52d and C-R2A-5d) belong to a single species. 16S rRNA gene-based phylogenetic analysis revealed that, together with Rhodobacter blasticus ATCC 33485T, the isolates form a deep line of descent (94.7–94.9 % sequence similarity) within the family Rhodobacteraceae loosely affiliated with the Rhodobacter/Paracoccus clade. The isolates were strictly aerobic and oxidase-positive (catalase was weakly positive) and utilized a wide range of substrates including pentoses, hexoses, oligosaccharides and sugar alcohols. The predominant constituents in their cellular fatty acid profiles were C19 : 0 cyclo ω8c (39–44 %), C18 : 0 (21–24 %) and C16 : 0 (21–23 %). Fatty acids present in smaller amounts included C18 : 1 ω7c, C10 : 0 3-OH, C18 : 1 ω7c 11-methyl, C20 : 2 ω6,9c and C17 : 0 cyclo, amongst others. Polar lipids included diphosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid, but not phosphatidylethanolamine. Carotenoid pigments were synthesized but bacteriochlorophyll a was not. The polyamine patterns consisted of the major compounds putrescine, spermidine and sym-homospermidine. The major respiratory lipoquinone was ubiquinone Q-10. The DNA G+C content was 69.4–70.2 mol%. On the basis of the phylogenetic and phenotypic evidence, the biofilm isolates were classified in a new genus, Rubellimicrobium gen. nov.; four of the isolates are assigned to the type species, Rubellimicrobium thermophilum gen. nov., sp. nov. Strain C-lvk-R2A-2T (=CCUG 51817T=DSM 16684T=HAMBI 2421T) is the type strain of Rubellimicrobium thermophilum.", }