1887

Abstract

Six red-pigmented strains of the with optimal growth between 45 and 54 °C were previously isolated from coloured biofilms in two fine-paper machines and one pulp dryer. The strains were found to be resistant to 15 p.p.m. 2,2-dibromo-3-nitrilopropionamide, a common industrial biocide. 16S RNA gene sequence similarity of the isolates was 99.7–100 %. Ribotyping using the restriction enzymes II and RI showed that four of the isolates (C-lvk-R2A-1, C-lvk-R2A-2, C-R2A-52d and C-R2A-5d) belong to a single species. 16S rRNA gene-based phylogenetic analysis revealed that, together with ATCC 33485, the isolates form a deep line of descent (94.7–94.9 % sequence similarity) within the family loosely affiliated with the clade. The isolates were strictly aerobic and oxidase-positive (catalase was weakly positive) and utilized a wide range of substrates including pentoses, hexoses, oligosaccharides and sugar alcohols. The predominant constituents in their cellular fatty acid profiles were C cyclo 8 (39–44 %), C (21–24 %) and C (21–23 %). Fatty acids present in smaller amounts included C 7, C 3-OH, C 7 11-methyl, C 6,9 and C cyclo, amongst others. Polar lipids included diphosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid, but not phosphatidylethanolamine. Carotenoid pigments were synthesized but bacteriochlorophyll was not. The polyamine patterns consisted of the major compounds putrescine, spermidine and -homospermidine. The major respiratory lipoquinone was ubiquinone Q-10. The DNA G+C content was 69.4–70.2 mol%. On the basis of the phylogenetic and phenotypic evidence, the biofilm isolates were classified in a new genus, gen. nov.; four of the isolates are assigned to the type species, gen. nov., sp. nov. Strain C-lvk-R2A-2 (=CCUG 51817=DSM 16684=HAMBI 2421) is the type strain of .

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2006-06-01
2019-12-13
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