1887

Abstract

A Gram-positive, rod- or coccus-shaped bacterial strain, KSL-104, was isolated from an alkaline soil from Korea and its taxonomic position was investigated by a polyphasic approach. Strain KSL-104 grew optimally at pH 7·0–8·0 and 30 °C. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on -2,6-diaminopimelic acid with MK-8(H) as the predominant menaquinone. The major fatty acid was iso-C and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The DNA G+C content was 70·6 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KSL-104 joined the cluster comprising and . The cellular fatty acid profile of strain KSL-104 was different from that of . Strain KSL-104 and the type strain of exhibited a 16S rRNA gene sequence similarity value of 97·1 % and a mean DNA–DNA relatedness value of 13 %. Levels of 16S rRNA gene sequence similarity between strain KSL-104 and the type strains of other species were in the range 94·0–96·5 %. On the basis of phenotypic, phylogenetic and genetic data, strain KSL-104 (=KCTC 9992=JCM 13008) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2005-09-01
2019-10-22
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