1887

Abstract

Strain LU14, isolated from the roots of , was characterized using a polyphasic approach. 16S rRNA gene sequence studies showed a similarity of 98·7 % to the corresponding sequence of DSM 15626. Chemotaxonomic data gathered for fatty acids, phospholipids, cell-wall peptidoglycan and menaquinones strongly supported the classification of this strain in the genus and DNA–DNA hybridization studies suggested that it may represent a novel species. Many physiological features were found that clearly distinguished isolate LU14 from other species. Based on the above data, a novel species of the genus , sp. nov., is proposed with the type strain LU14 (=DSM 16683=LMG 22957).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63745-0
2006-02-01
2020-12-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/2/407.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63745-0&mimeType=html&fmt=ahah

References

  1. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp  21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  6. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2 - molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  7. Lechevalier M. P., De Bièvre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  8. Lee S. D., Kang S. O., Hah Y. C. 2000; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  9. Li W.-J., Wang D., Zhang Y.-Q., Schumann P., Stackebrandt E., Xu L.-H., Jiang C.-L. 2004; Kribbella antibiotica sp. nov., a novel nocardioform actinomycete strain isolated from soil in Yunnan, China. Syst Appl Microbiol 27:160–165 [CrossRef]
    [Google Scholar]
  10. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  12. Park Y.-H., Yoon J.-H., Shin Y. K., Suzuki K., Kudo T., Seino A., Kim H.-J., Lee J. S., Lee S. T. 1999; Classification of ‘ Nocardioides fulvus ’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen nov., as Kribbella flavida sp.nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49:743–752 [CrossRef]
    [Google Scholar]
  13. Rivas R., Sánchez M., Trujillo M. E., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Velázquez E. 2003; Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree ( Ulmus nigra ). Int J Syst Evol Microbiol 53:99–103 [CrossRef]
    [Google Scholar]
  14. Romanenko L. A., Lysenko A. M., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54:1741–1745 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  17. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  18. Schröder K. H., Naumann L., Kroppenstedt R. M., Reischl U. 1997; Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. Int J Syst Bacteriol 47:86–91 [CrossRef]
    [Google Scholar]
  19. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  20. Sohn K., Hong S. G., Bae K. S., Chun J. 2003; Transfer of Hongia koreensis Lee et al . 2000 to the genus Kribbella Park et al . 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 53:1005–1007 [CrossRef]
    [Google Scholar]
  21. Song J., Kim B.-Y., Hong S.-B., Cho H.-S., Sohn K., Chun J., Suh J.-W. 2004; Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea. Int J Syst Evol Microbiol 54, 1345–1348 [CrossRef]
    [Google Scholar]
  22. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  24. Trujillo M. E., Fernández-Molinero C., Velázquez E., Kroppenstedt R. M., Schumann P., Mateos P. F., Martínez-Molina E. 2005; Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55:877–880 [CrossRef]
    [Google Scholar]
  25. Vincent J. M. 1970; The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria pp  1–13 Edited by Vincent J. M. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  26. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  27. Wink J. M., Kroppenstedt R. M., Ganguli B. N., Nadkarni S. R., Schumann P., Seibert G., Stackebrandt E. 2003; Three new antibiotic producing species of the genus Amycolatopsis , Amycolatopsis balhimycina sp.nov., A. tolypomycina sp. nov., A. vancoresmycina sp.nov., and description of Amycolatopsis keratiniphila subsp. keratiniphila subsp.nov. and A.keratiniphila subsp. nogabecina subsp. nov. Syst Appl Microbiol 26:38–46 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63745-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63745-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error