A Gram-negative, aerobic and halophilic bacterium designated strain mano18 was isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. This strain was motile by means of polar flagella, occasionally forming rosette-like aggregates, reduced nitrate to nitrite, required sodium ions for growth, exhibited catalase and oxidase activities and contained Q-10 as the major quinone and C 7 as the dominant cellular fatty acid. Analysis of the 16S rRNA gene sequence revealed that this strain is affiliated with a cluster within the . Strain mano18 synthesized bacteriochlorophyll under aerobic conditions. The 16S rRNA gene sequence similarity between strain mano18 and the most closely related species, DSM 13394, was 98·5 %. Levels of DNA–DNA relatedness between strain mano18 and the type strains of and were respectively 6·2–11·2 and 3·3–7·6 %. Strain mano18, like other strains, possesses carbon monoxide dehydrogenase genes. The results of DNA–DNA hybridization and the polyphasic data confirmed that strain mano18 can be considered to represent a novel taxon in the genus . The name sp. nov. is proposed for the tidal flat isolate; the type strain is strain mano18 (=KCTC 12288=DSM 17023).


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