strains recently isolated from French ‘framboisé’ ciders were compared with collection strains of the two defined subspecies, subsp. and subsp. , using a polyphasic approach. Six strains isolated from six different regions of France were compared with three strains of subsp. , including the type strain LMG 404, and four strains of subsp. , including the type strain LMG 448, using phenotypic and genotypic methods. For phenotypic characterization, both physiological tests and SDS-PAGE protein profiles revealed significant differences between the two known subspecies and the French isolates; three distinct groups were observed. These findings were further confirmed by random amplified polymorphic DNA and repetitive extragenic palindromic-PCR genotyping methods in which the French isolates were clearly distinguished from the other two subspecies. Sequence analysis of a fragment ranging from 604 to 617 nucleotides corresponding to the 16S–23S rRNA gene intergenic spacer region (ISR), a 592 nucleotide HSP60 gene fragment and a 1044 nucleotide gene fragment confirmed the presence of three distinct groups. The French strains exhibited almost 94 % similarity to the ISR, 90 % to HSP60 and 86 % to sequences of the three collection strains of subsp. and 87, 84 and 80 % sequence similarity, respectively, was observed with the four subsp. strains. Based on both the phenotypic and genotypic results, the French strains are proposed to represent a novel subspecies, subsp. subsp. nov. Strain AN0101 (=LMG 22974=CIP 108684) was designated as the type strain.


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vol. , part 1, pp. 121 - 125

Figures showing the results of SDS-PAGE analysis and additional phylogenetic trees are available as [PDF](317 KB)


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