1887

Abstract

A strictly anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated strain R1, was isolated from bovine rumen fluid and characterized. Cells of this isolate were Gram-positive, non-motile rods that formed spherical terminal spores. The overall biochemical and physiological characteristics indicated that this strain should be placed in the genus . The strain grew at temperatures between 25 and 47 °C (optimum, 37 °C), at pH between 5·0 and 8·5 (optimum pH 5·5–7·0) and in NaCl concentrations of 0–3 % (w/v). The isolate was not able to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31·2 mol%. Sequence analysis of the 16S rRNA gene of R1 revealed that it has the closest match (98 % similarity) with DSM 4474. The highest levels of DNA–DNA relatedness of the isolate were 61·9 and 54·3 % with DSM 10365 and DSM 4474, respectively. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA–DNA hybridization data and distinct phenotypic characteristics, strain R1 (=DSM 17049=CCUG 50446) was classified in the genus , as a member of a novel species, for which the name sp. nov. is proposed.

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2006-03-01
2020-09-24
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