1887

Abstract

Two enterococcal strains LMG 16607 and LMG 16612 originating from sea water were analysed in a polyphasic taxonomic study. Both strains, assigned as sp. in the BCCM/LMG culture collection, possessed analogous protein profiles, but these were different from all other enterococcal species. 16S rRNA gene sequence analysis of one strain showed the highest similarity, 96·9–96·1 %, with its closest phylogenetic neighbours , , and . Further genomic analysis by (GTG)-PCR fingerprinting and sequence analysis of the housekeeping gene phenylalanyl-tRNA synthase () and distinct biochemical features confirmed that the two strains represent a novel enterococcal species for which the name sp. nov. is proposed. The type strain is LMG 16607 (=CCM 7283).

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2005-09-01
2019-10-21
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References

  1. De Graef, E. M., Devriese, L. A., Vancanneyt, M., Baele, M., Collins, M. D., Lefebvre, K., Swings, J. & Haesebrouck, F. ( 2003; ). Description of Enterococcus canis sp. nov. from dogs and reclassification of Enterococcus porcinus Teixeira et al. 2001 as a junior synonym of Enterococcus villorum Vancanneyt et al. 2001. Int J Syst Evol Microbiol 53, 1069–1074.[CrossRef]
    [Google Scholar]
  2. de Vaux, A., Laguerre, G., Divies, C. & Prevost, H. ( 1998; ). Enterococcus asini sp. nov. isolated from the caecum of donkeys (Equus asinus). Int J Syst Bacteriol 48, 383–387.[CrossRef]
    [Google Scholar]
  3. Devriese, L. A. & Pot, B. ( 1995; ). The genus Enterococcus. In The Genera of Lactic Acid Bacteria, pp. 327–367. Edited by B. J. B. Wood & W. H. Holzapfel. London: Blackie Academic & Professional.
  4. Devriese, L. A., Collins, M. D. & Wirth, R. ( 1992; ). The genus Enterococcus. In The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, pp. 1465–1481. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  5. Devriese, L. A., Pot, B. & Collins, M. D. ( 1993; ). Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol 75, 399–408.[CrossRef]
    [Google Scholar]
  6. Devriese, L. A., Vancanneyt, M., Descheemaeker, P., Baele, M., Van Landuyt, H. W., Gordts, B., Butaye, P., Swings, J. & Haesebrouck, F. ( 2002; ). Differentiation and identification of Enterococcus durans, E. hirae and E. villorum. J Appl Microbiol 92, 821–827.[CrossRef]
    [Google Scholar]
  7. Domig, K. J., Mayer, H. K. & Kneifel, W. ( 2003; ). Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp. 2. Pheno- and genotypic criteria. Int J Food Microbiol 88, 165–188.[CrossRef]
    [Google Scholar]
  8. Farrow, J. A. E., Kruze, J., Phillips, B. A., Bramley, A. J. & Collins, M. D. ( 1984; ). Taxonomic studies on Streptococcus bovis and Streptococcus equinus: description of Streptococcus alactolyticus sp. nov. and Streptococcus saccharolyticus sp. nov. Syst Appl Microbiol 5, 467–482.[CrossRef]
    [Google Scholar]
  9. Fortina, M. G., Ricci, G., Mora, D. & Manachini, P. L. ( 2004; ). Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov. Int J Syst Evol Microbiol 54, 1717–1721.[CrossRef]
    [Google Scholar]
  10. Gevers, D., Huys, G. & Swings, J. ( 2001; ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205, 31–36.[CrossRef]
    [Google Scholar]
  11. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  12. Mannerová, S., Pantůček, R., Doškař, J., Švec, P., Snauwaert, C., Vancanneyt, M., Swings, J. & Sedláček, I. ( 2003; ). Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas. Int J Syst Evol Microbiol 53, 1647–1654.[CrossRef]
    [Google Scholar]
  13. Martinez-Murcia, A. J. & Collins, M. D. ( 1991; ). Enterococcus sulfureus, a new yellow-pigmented Enterococcus species. FEMS Microbiol Lett 80, 69–73.[CrossRef]
    [Google Scholar]
  14. Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M. & Swings, J. ( 2005; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151, 2141–2150.[CrossRef]
    [Google Scholar]
  15. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbiological Methods: Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  16. Raes, J. & Van de Peer, Y. ( 1999; ). ForCon: a software tool for the conversion of sequence alignments. http://www.ebi.ac.uk/embnet.news/vol6_1/ForCon/body_forcon.html
  17. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  18. Švec, P., Devriese, L. A., Sedláček, I., Baele, M., Vancanneyt, M., Haesebrouck, F., Swings, J. & Doškař, J. ( 2001; ). Enterococcus haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water. Int J Syst Evol Microbiol 51, 1567–1574.
    [Google Scholar]
  19. Vancanneyt, M., Zamfir, M., Devriese, L. A. & 7 other authors ( 2004; ). Enterococcus saccharominimus sp. nov., from dairy products. Int J Syst Evol Microbiol 54, 2175–2179.[CrossRef]
    [Google Scholar]
  20. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
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vol. , part 5, pp. 2183 - 2187

Neighbour-joining tree based on the gene sequences of all enterococcal type strains. [PDF](177 KB)



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