1887

Abstract

Two gamma- and UV-radiation-tolerant, Gram-negative, rod-shaped bacterial strains, VCD115 and VCD117, were isolated from a mixture of sand samples collected in the Sahara Desert in Morocco and Tunisia, after exposure of the sand to 15 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridizations showed that VCD115 and VCD117 are members of a novel species belonging to the genus , with as its closest relative. The DNA G+C contents of VCD115 and VCD117 are 59·8 and 60·6 mol%, respectively. The major fatty acids (straight-chain 15 : 1, 16 : 1, 17 : 1 and 16 : 0), polar lipids (dominated by phosphoglycolipids and glycolipids) and quinone type (MK-8) support the affiliation to the genus . The strains did not grow on rich medium such as trypticase soy broth (TSB), but did grow as whitish colonies on tenfold-diluted TSB. The genotypic and phenotypic properties allowed differentiation of VCD115 and VCD117 from recognized species. Strains VCD115 and VCD117 are therefore identified as representing a novel species, for which the name sp. nov. is proposed, with the type strain VCD115 (=DSM 17065=LMG 22923).

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2005-11-01
2024-11-11
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References

  1. Brim H., Venkateswaran A., Kostandarithes H. M., Fredrickson J. K., Daly M. J. 2003; Engineering Deinococcus geothermalis for bioremediation of high-temperature radioactive waste environments. Appl Environ Microbiol 69:4575–4582 [CrossRef]
    [Google Scholar]
  2. Earl A. M., Mohundro M. M., Mian I. S., Battista J. R. 2002; The IrrE protein of Deinococcus radiodurans R1 is a novel regulator of recA expression. J Bacteriol 184:6216–6224 [CrossRef]
    [Google Scholar]
  3. Edwards J. S., Battista J. R. 2003; Using DNA microarray data to understand the ionizing radiation resistance of Deinococcus radiodurans . Trends Biotechnol 21:381–382 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Habuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [CrossRef]
    [Google Scholar]
  6. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win, two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
    [Google Scholar]
  7. Gascuel O. 1997; bionj, an improved version of the NJ algorithm based on a simple method of sequence data. Mol Biol Evol 14:685–695 [CrossRef]
    [Google Scholar]
  8. Hirsch P., Gallikowski C. A., Siebert J., Peissl K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R. 2004; Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27:636–645 [CrossRef]
    [Google Scholar]
  9. Lipton M. S., Paša-Tolič L., Anderson G. A. 18 other authors 2002; Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags. Proc Natl Acad Sci U S A 99:11049–11054 [CrossRef]
    [Google Scholar]
  10. Liu Y., Zhou J., Omelchenko M. V. 12 other authors 2003; Transcriptome dynamics of Deinococcus radiodurans recovering from ionizing radiation. Proc Natl Acad Sci U S A 100:4191–4196 [CrossRef]
    [Google Scholar]
  11. Makarova K. S., Aravind L., Wolf Y. I., Tatusov R. L., Minton K. W., Koonin E. V., Daly M. J. 2001; Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol Rev 65:44–79 [CrossRef]
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  13. Mattimore V., Battista J. R. 1996; Radioresistance of Deinococcus radiodurans : functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637
    [Google Scholar]
  14. Narumi I. 2003; Unlocking radiation resistance mechanisms: still a long way to go. Trends Microbiol 11:422–425 [CrossRef]
    [Google Scholar]
  15. Narumi I., Cherdchu K., Kitayama S., Watanabe H. 1997; The Deinococcus radiodurans uvrA gene: identification of mutation sites in two mitomycin-sensitive strains and the first discovery of insertion sequence element from deinobacteria. Gene 198:115–126 [CrossRef]
    [Google Scholar]
  16. Owen R. J., Lapage S. P. 1976; The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic implications. J Appl Bacteriol 41:335–340 [CrossRef]
    [Google Scholar]
  17. Perrière G., Gouy M. 1996; www-query, an on-line retrieval system for biological sequence banks. Biochimie 78:364–369 [CrossRef]
    [Google Scholar]
  18. Rainey F. A., Nobre M. F., Schumann P., Stackebrandt E., da Costa M. S. 1997; Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47:510–514 [CrossRef]
    [Google Scholar]
  19. Suresh K., Reddy G. S., Sengupta S., Shivaji S. 2004; Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54:457–461 [CrossRef]
    [Google Scholar]
  20. Tanaka M., Earl A. M., Howell H. A., Park M. J., Eisen J. A., Peterson S. N., Battista J. R. 2004; Analysis of Deinococcus radiodurans 's transcriptional response to ionizing radiation and desiccation reveals novel proteins that contribute to extreme radioresistance. Genetics 168:21–33 [CrossRef]
    [Google Scholar]
  21. Thompson B. G., Anderson R., Murray R. G. 1980; Unusual polar lipids of Micrococcus radiodurans strain Sark. Can J Microbiol 26:1408–1411 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. G., Coldwell R. R. 8 other authors 1987; Report of the ad hoc committee on approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Weaver P. F., Wall J. D., Gest H. 1975; Characterization of Rhodopseudomonas capsulata . Arch Microbiol 105:207–216 [CrossRef]
    [Google Scholar]
  24. White O., Eisen J. A., Heidelberg J. F. 29 other authors 1999; Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286:1571–1577 [CrossRef]
    [Google Scholar]
  25. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
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