1887

Abstract

Two gamma- and UV-radiation-tolerant, Gram-negative, rod-shaped bacterial strains, VCD115 and VCD117, were isolated from a mixture of sand samples collected in the Sahara Desert in Morocco and Tunisia, after exposure of the sand to 15 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridizations showed that VCD115 and VCD117 are members of a novel species belonging to the genus , with as its closest relative. The DNA G+C contents of VCD115 and VCD117 are 59·8 and 60·6 mol%, respectively. The major fatty acids (straight-chain 15 : 1, 16 : 1, 17 : 1 and 16 : 0), polar lipids (dominated by phosphoglycolipids and glycolipids) and quinone type (MK-8) support the affiliation to the genus . The strains did not grow on rich medium such as trypticase soy broth (TSB), but did grow as whitish colonies on tenfold-diluted TSB. The genotypic and phenotypic properties allowed differentiation of VCD115 and VCD117 from recognized species. Strains VCD115 and VCD117 are therefore identified as representing a novel species, for which the name sp. nov. is proposed, with the type strain VCD115 (=DSM 17065=LMG 22923).

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2005-11-01
2019-10-19
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Supplements

vol. , part 6, pp. 2441 - 2446

Levels of 16S rRNA gene sequence similarity and DNA–DNA hybridization.

Survival rates after exposure to gamma and UV radiation.

[PDF of Supplementary Tables S1 and S2](14 KB)



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