1887

Abstract

A novel xylan-degrading bacterium, S3-E, was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that were consistent with classification in the genus , i.e. MK-12, MK-11 and MK-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as the cell-wall sugars, peptidoglycan-type B2 with glycolyl residues and a DNA G+C content of 69·7 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain S3-E is most similar to IFO 15708 and DSM 12966 (97·6 and 97·4 % sequence similarity, respectively), and that it forms a separate lineage with in the genus . DNA–DNA hybridization results and phenotypic properties showed that strain S3-E could be distinguished from all known species and represented a novel species, for which the name sp. nov. is proposed; the type strain is S3-E (=DSM 16914=KCTC 19079).

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2005-09-01
2019-09-18
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Phase-contrast micrograph of cells of strain S3-E . Bar, 5 µm.

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16S rRNA gene phylogenetic tree, obtained using the neighbour-joining method, showing the position of strain S3-E relative to all species with validly published names. [PDF](1069 KB)

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