The taxonomic position of two spore-forming strains 6T19 and 6T29, isolated from cotton composts for the cultivation of oyster mushroom (), was investigated by a polyphasic approach. Cells of strains 6T19 and 6T29 were rod-shaped, Gram-negative and strictly aerobic. Sequencing and comparative analyses for the 16S rRNA genes of these strains clearly showed their phylogenetic affiliation to the genus . Their closest relatives and have sequence similarity of 96·9 and 97·5 %, respectively. The isoprenoid quinones of isolate 6T19 were MK-9, MK-8, MK-7, MK-10 and MK-6 (45 : 27 : 18 : 5 : 4 %), the peptidoglycan type was -lys←-Asp and the main cellular fatty acid was i-C. DNA–DNA hybridization experiments resulted in relatedness values of 37 % between 6T19 and DSM 10633 and 41 % between 6T19 and DSM 12654. Based on the polyphasic data, strains 6T19 and 6T29 can be described as members of a novel species of the genus s, for which the name sp. nov. is proposed. The type strain is 6T19 (=KACC 11287=DSM 16752).


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  1. Atlas, R. M. ( 1993; ). In Handbook of Microbiological Media, p. 967. Edited by L. C. Parks. London: CRC Press.
  2. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  3. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Fortina, M. G., Pukall, R., Schumann, P., Mora, D., Parini, C., Manachini, P. L. & Stackebrandt, E. ( 2001; ). Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Anderson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov. Int J Syst Evol Microbiol 51, 447–455.
    [Google Scholar]
  5. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N. ( 1973; ). The Genus Bacillus. Agriculture Handbook no. 427. Washington, DC: US Department of Agriculture.
  6. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  7. Heimbrook, M. E., Wang, W. L. L. & Campbell, G. ( 1989; ). Staining bacterial flagella easily. J Clin Microbiol 27, 2612–2615.
    [Google Scholar]
  8. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  12. MacFaddin, J. F. ( 2000; ). Urease test. In Biochemical Tests for Identification of Medical Bacteria, 3rd edn, pp. 424–438. Baltimore: Lippincott Williams & Wilkins.
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]

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vol. , part 3, pp. 663 - 666

Morphological shapes of strain 6T19

Cellular fatty-acid composition of and other species [Single PDF file](226 KB)


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