Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 1

sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea Free

Abstract

An endospore-forming, rod-shaped bacterium was isolated from the rhizosphere of willow roots in Korea. The bacterium, designated strain BR030, was a strictly aerobic, motile rod. The cell wall contained type A1 peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were anteiso-C, iso-C, iso-C and iso-C. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and unknown phospholipids (PL1, PL2). The genomic DNA G+C content was 36 mol%. Comparative 16S rRNA gene sequence analyses showed that strain BR030 formed a distinct phyletic line within the genus and was most closely related to DSM 1320, with 16S rRNA gene sequence similarity of 96·8 %. Sequence similarities to other type strains were lower than 96·2 %. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BR030 (=KCTC 3914=DSM 16467).

  • Published Online:
Keyword(s): m-DAP, meso-diaminopimelic acid and TEM, transmission electron microscopy
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63701-0
2006-01-01
2025-07-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/59.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63701-0&mimeType=html&fmt=ahah

References

  1. Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D.(1991). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. Lett Appl Microbiol 13, 202–206. [Google Scholar]
  2. Bakermans, C. & Madsen, E. L.(2002). Diversity of 16S rDNA and naphthalene dioxygenase genes from coal-tar-waste-contaminated aquifer waters. Microb Ecol 44, 95–106. [Google Scholar]
  3. Bashan, Y., Holguin, G. & de Bashan, L. E.(2004).Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997–2003). Can J Microbiol 50, 521–577.[CrossRef] [Google Scholar]
  4. Berge, O., Guinebretière, M.-H., Achouak, W., Normand, P. & Heulin, T.(2002).Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52, 607–616. [Google Scholar]
  5. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  6. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Heyrman, J., Vanparys, B., Logan, N. A., Balcaen, A., Rodriguez-Diaz, M., Felske, A. & De Vos, P.(2004).Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54, 47–57.[CrossRef] [Google Scholar]
  8. Heyrman, J., Logan, N. A., Rodríguez-Díaz, M., Scheldeman, P., Lebbe, L., Swings, J., Heyndrickx, M. & De Vos, P.(2005). Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int J Syst Evol Microbiol 55, 119–131.[CrossRef] [Google Scholar]
  9. Jeon, C. O., Lee, D. S. & Park, J. M.(2003). Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Water Res 37, 2195–2205.[CrossRef] [Google Scholar]
  10. Jeon, C. O., Lim, J.-M., Park, D.-J. & Kim, C.-J.(2005).Salinimonas chungwhensis gen. nov., sp. nov., a moderately halophilic bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 239–243.[CrossRef] [Google Scholar]
  11. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  12. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–208. [Google Scholar]
  13. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  14. Lee, E. M., Jeon, C. O., Choi, I., Chang, K.-S. & Kim, C.-J.(2005).Silanimonas lenta gen. nov., sp. nov., a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 55, 385–389.[CrossRef] [Google Scholar]
  15. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  16. Lim, J.-M., Jeon, C. O., Park, D.-J., Kim, H.-R., Yoon, B.-J. & Kim, C.-J.(2005).Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus. Int J Syst Evol Microbiol 55, 1027–1031.[CrossRef] [Google Scholar]
  17. Nagel, M. & Andreesen, J. R.(1991).Bacillus niacini sp. nov., a nicotinate-metabolizing mesophile isolated from soil. Int J Syst Bacteriol 41, 134–139.[CrossRef] [Google Scholar]
  18. Nielsen, P., Rainey, F. A., Outtrup, H., Priest, F. G. & Fritze, D.(1994). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol Lett 117, 61–65.[CrossRef] [Google Scholar]
  19. Nielsen, P., Fritze, D. & Priest, F. G.(1995). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef] [Google Scholar]
  20. Penrose, D. M. & Glick, B. R.(2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant 118, 10–15.[CrossRef] [Google Scholar]
  21. Pettersson, B., Lembke, F., Hammer, P., Stackebrandt, E. & Priest, F. G.(1996).Bacillus sporothermodurans, a new species producing highly heat-resistant endospores. Int J Syst Bacteriol 46, 759–764.[CrossRef] [Google Scholar]
  22. Priest, F. G., Goodfellow, M. & Todd, C.(1988). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882. [Google Scholar]
  23. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  24. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M.(2001).Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431. [Google Scholar]
  25. Sessitsch, A., Reiter, B. & Berg, G.(2004). Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities. Can J Microbiol 50, 239–249.[CrossRef] [Google Scholar]
  26. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  27. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Somers, E., Vanderleyden, J. & Srinivasan, M.(2004). Rhizosphere bacterial signalling: a love parade beneath our feet. Crit Rev Microbiol 30, 205–240.[CrossRef] [Google Scholar]
  29. Stackebrandt, E. & Liesack, W.(1993). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  30. Suresh, K., Prabagaran, S. R., Sengupta, S. & Shivaji, S.(2004).Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54, 1369–1375.[CrossRef] [Google Scholar]
  31. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  32. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  33. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  34. Yumoto, I., Hirota, K., Yamaga, S., Nodasaka, Y., Kawasaki, T., Matsuyama, H. & Nakajima, K.(2004).Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54, 1997–2001.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63701-0
Loading
Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea
Int J Syst Evol Microbiol 56, 59 (2006); https://doi.org/10.1099/ijs.0.63701-0
/content/journal/ijsem/10.1099/ijs.0.63701-0
/content/journal/ijsem/10.1099/ijs.0.63701-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 59 - 63

[PDF](105 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error