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Volume 56, Issue 4

sp. nov., sp. nov. and sp. nov., isolated from brewery samples, and emended description of the genus Free

Abstract

Seven unidentified strictly anaerobic, Gram-negative, non-spore-forming bacteria from spoiled beer or the brewery environment were characterized. Based on 16S rRNA gene sequence analyses, all strains were affiliated to the sub-branch of the class ‘’. Three of the strains were non-motile cocci, on average 1·5×1·2 μm or 1·2×1·0 μm, occurring mainly singly or in pairs. They shared nearly identical (>99 %) 16S rRNA gene sequences, being most closely related to the species of the group (⩽93·9 % similarity). According to DNA–DNA hybridization results, the coccoid strains represented two genospecies, neither of which was related to any of the recognized species. Several phenotypic characteristics and/or DNA G+C content also differentiated the strains from each other and from their closest relatives. The other four novel strains were motile, slightly curved to helical rods, 0·6−0·8×3−50 μm or more in size. They shared identical 16S rRNA gene sequences and ribofragment patterns. The highest 16S rRNA gene similarity was found between these isolates and ATCC 29359 (95·6 %) and ATCC 33332 (93·6 %). The novel strains also differed from recognized species in their sugar utilization, proteolytic activity, catalase activity, antibiotic resistance and temperature tolerance. The results suggest that the bacteria belong to three novel species, for which the names sp. nov. (type strain VTT E-032341=DSM 16981), sp. nov. (type strain VTT E-97791=DSM 17042) and sp. nov. (type strain VTT E-88329=DSM 16980) are proposed.

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Keyword(s): ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
SGM
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2006-04-01
2024-04-23
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  2. Amann R. I., Lin C., Key R., Montgomery L., Stahl D. A. 1992; Diversity among Fibrobacter strains: towards a phylogenetic classification. Syst Appl Microbiol 15:23–31
     [Google Scholar]
  3. Bruce J. 1996; Automated system rapidly identifies and characterizes micro-organisms in food. Food Technol 50:77–81
     [Google Scholar]
  4. Carlier J.-P., Marchandin H., Jumas-Bilak E., Lorin V., Henry C., Carrière C., Jean-Pierre H. 2002; Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae . Int J Syst Evol Microbiol 52:983–986 [CrossRef]
     [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
     [Google Scholar]
  7. Engelmann U., Weiss N. 1985; Megasphaera cerevisiae sp. nov.: a new gram-negative obligately anaerobic coccus isolated from spoiled beer. Syst Appl Microbiol 6:287–290 [CrossRef]
     [Google Scholar]
  8. Gutierrez J., Davis R. E., Lindahl I. L., Warwick E. J. 1959; Bacterial changes in the rumen during the onset of feed-lot bloat of cattle and characteristics of Peptostreptococcus elsdenii n. sp. Appl Microbiol 7:16–22
     [Google Scholar]
  9. Haikara A. 1989; Invasion of anaerobic bacteria into pitching yeast. In Proceedings of the European Brewery Convention , 22nd edn. pp  537–544 New York: Oxford University Press;
     [Google Scholar]
  10. Haikara A., Helander I. 2002 Pectinatus , Megasphaera and Zymophilus . In The Prokaryotes: an Evolving Electronic Database for the Microbiological Community , 3rd edn, release 3.5. Edited by Dworkin M. New York: Springer;
     [Google Scholar]
  11. Haikara A., Lounatmaa K. 1987; Characterization of Megasphaera sp., a new anaerobic beer spoilage coccus. In Proceedings of the European Brewery Convention , 21st edn. pp  473–480 New York: Oxford University Press;
     [Google Scholar]
  12. Haikara A., Penttilä L., Enari T.-M., Lounatmaa K. 1981; Microbiological, biochemical, and electron microscopic characterization of a Pectinatus strain. Appl Environ Microbiol 41:511–517
     [Google Scholar]
  13. Hespell R. B., Paster B. J., Dewhirst F. E. 1999 The genus Selenomonas . In The Prokaryotes: an Evolving Electronic Database for the Microbiological Community , 3rd edn, release 3.0. Edited by Dworkin M. New York: Springer;
     [Google Scholar]
  14. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
     [Google Scholar]
  15. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
     [Google Scholar]
  16. Jousimies-Somer H.-R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M. 2002 Wadsworth – KTL Anaerobic Bacteriology Manual , 6th edn. Belmont, CA: Star Publishing;
     [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. H. New York: Academic Press;
     [Google Scholar]
  18. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
     [Google Scholar]
  19. Lee S. Y. 1994; SMMP – a medium for selective isolation of Megasphaera and Pectinatus from the brewery. J Am Soc Brew Chem 52:115–119
     [Google Scholar]
  20. Lee S. Y., Mabee M. S., Jangaard N. O. 1978; Pectinatus , a new genus of the family Bacteroidaceae . Int J Syst Bacteriol 28:582–594 [CrossRef]
     [Google Scholar]
  21. Marchandin H., Jumas-Bilak E., Gay B., Teyssier C., Jean-Pierre H., Siméon de Buochberg M., Carrière C., Carlier J. P. 2003; Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov. Int J Syst Evol Microbiol 53:547–553 [CrossRef]
     [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  23. Olsen G. J., Matsuda H., Hagström R., Overbeek R. 1994; fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
     [Google Scholar]
  24. Rajakylä E. 1981; Separation and determination of some organic acids and their sodium salts by high-performance liquid chromatography. J Chromatogr 218:695–701 [CrossRef]
     [Google Scholar]
  25. Rogosa M. 1971; Transfer of Peptostreptococcus elsdenii Gutierrez et al . to a new genus,Megasphaera [ M. elsdenii (Gutierrez et al. ) comb. nov.]. Int J Syst Bacteriol 21:187–189 [CrossRef]
     [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  27. Sakamoto K. 1997; Detection of bacterium belonging to the genus Pectinatus . European patent EP0806483:A1
     [Google Scholar]
  28. Schleifer K. H., Leuteritz M., Weiss N., Ludwig W., Kirchhof G., Seidel-Rüfer H. 1990; Taxonomic study of anaerobic, gram-negative, rod-shaped bacteria from breweries: emended description of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp.nov., Selenomonas lacticifex sp. nov., Zymophilus raffinosivorans gen. nov., sp. nov., and Zymophilus paucivorans sp. nov. Int J Syst Bacteriol 40:19–27 [CrossRef]
     [Google Scholar]
  29. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  30. Strunk O., Gross O., Reichel B. & 10 other authors 2000 arb: a software environment for sequence data Department of Microbiology, Technische Universität München; Munich, Germany: http://www.arb-home.de
     [Google Scholar]
  31. Strömpl C., Tindall B. J., Jarvis G. N., Lünsdorf H., Moore E. R. B., Hippe H. 1999; A re-evaluation of the genus Anaerovibrio , with the reclassification of Anaerovibrio glycerini as Anaerosinus glycerini gen.nov., comb. nov. and Anaerovibrioburkinabensis as Anaeroarcus burkinensis [corrig.] gen. nov., comb. nov. Int J Syst Bacteriol 49, 1861–1872 [CrossRef]
     [Google Scholar]
  32. Suihko M.-L. 1999; VTT Culture Collection Catalogue of Strains . , 4th edn. Espoo, Finland: Libella Painopalvelu Oy;
     [Google Scholar]
  33. Suihko M.-L., Haikara A. 2001; Characterization of Pectinatus and Megasphaera strains by automated ribotyping. J Inst Brew 107:175–184 [CrossRef]
     [Google Scholar]
  34. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  36. Willems A., Collins M. D. 1995; Phylogenetic placement of Dialister pneumosintes (formerly Bacteroides pneumosintes ) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int J Syst Bacteriol 45:403–405 [CrossRef]
     [Google Scholar]
  37. Wilmotte A., Van der Auwera G., De Wachter R. 1993; Structure of the 16S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘ Mastigocladus laminosus HTF’) strain PCC7518, and phylogenetic analysis. FEBS Lett 317:96–100 [CrossRef]
     [Google Scholar]
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Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus
Int J Syst Evol Microbiol 56, 695 (2006); https://doi.org/10.1099/ijs.0.63699-0
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