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Abstract

A Gram-negative bacterium, YIM 003, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7·0–7·5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C 7 (59·8 %), C (9·9 %), ai-C (5·3 %), i-C (4·4 %) and C 2-OH (15·8 %). The G+C content of the genomic DNA was 67·5 mol%. Strain YIM 003 exhibited levels of 16S rRNA gene sequence similarity of 98·2 % to FA2 and 98·0 % to DSM 7418 but showed less than 97·0 % similarity with respect to other species with validly published names. The DNA–DNA relatedness values of the isolate with FA2 and DSM 7418 were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003 should be distinguished from FA2 and DSM 7418. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus , sp. nov., is proposed, with the type strain YIM 003 (=CCTCC AB 204064=KCTC 12346).

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2005-11-01
2020-01-21
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vol. , part 6, pp. 2361 - 2364

Two-dimensional thin-layer chromatogram of the phosphate-containing lipids of strain YIM 003 , obtained using molybenum blue as the spray reagent. [PDF](35 KB)



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