1887

Abstract

A moderately thermophilic, anaerobic bacterium (strain SB91) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91 were straight to slightly curved rods, 0·5–0·6 μm in diameter and 3·0–7·0 μm in length. Formation of endospores was not observed. The temperature range for growth was 26–62 °C, with an optimum at 50 °C. The pH range for growth was 5·5–9·5, with an optimum at pH 7·5–8·0. The substrates utilized by strain SB91 in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, -valine and n-propanol. Carbohydrates were not utilized. Strain SB91 reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, -valine or n-propanol as an electron donor. Strain SB91 reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91 survived after exposure to gamma-radiation at a dose of 5·4 kGy. The G+C content of the DNA of strain SB91 was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91 represents the sole species of a novel genus, ; the name gen. nov., sp. nov. is proposed, with strain SB91 (=DSM 16624=VKM B-2348) as the type strain.

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2006-02-01
2019-12-13
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